Effects of Chlorich®EnergyBoost on Enhancing Physical Performance and Anti-Fatigue Properties in Mice

Foods Pub Date : 2024-07-16 DOI:10.3390/foods13142232
Shih-An Yang, Po-Hsun Cheng, Yi-Ju Hsu, Shu-Feng Cheng, Meng-Hsueh Amanda Lin, Chi-Chang Huang
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Abstract

Chlorich®EnergyBoost, a water extract obtained from Chlorella sorokiniana, has been proposed to enhance physical performance and provide anti-fatigue effects. This study assessed the impact of Chlorich®EnergyBoost supplementation on physical performance and its anti-fatigue properties. Twenty-four mice were allocated into four groups: (1) the control group receiving only water,;(2) the 1X group (49.2 mg/kg/day); (3) the 2X group (98.4 g/kg/day); and (4) the 5X group (246 g/kg/day). All groups were orally administered the supplements for four consecutive weeks. The evaluation included grip strength, swimming endurance, an exhaustion test, and serum biochemistry analysis. Additionally, the study examined the bioactive peptides through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) and conducted bacterial reverse mutation and acute oral toxicity tests for safety assessment. The findings indicated that Chlorich®EnergyBoost supplementation led to a significant reduction in serum lactate levels by 14.08% to 22.54% and blood urea nitrogen levels by 12.23% to 16.76%, an increase in the lactate clearance rate by 0.28 to 0.35, an enhancement of muscle glycogen storage by 1.10 to 1.44-fold, and hepatic glycogen storage by 1.41 to 1.47-fold. These results demonstrated dose-dependent effects. MALDI-TOF analysis revealed the expression of dihydrolipoamide dehydrogenase and superoxide dismutase. Both the bacterial reverse mutation and acute oral toxicity tests showed no adverse effects.
Chlorich®EnergyBoost 对提高小鼠体能和抗疲劳性能的影响
Chlorich®EnergyBoost 是一种从小球藻(Chlorella sorokiniana)中提取的水提取物,被认为可以提高体能和抗疲劳效果。本研究评估了补充 Chlorich®EnergyBoost 对体能表现的影响及其抗疲劳特性。24 只小鼠被分为四组:(1) 只喝水的对照组;(2) 1X 组(49.2 毫克/千克/天);(3) 2X 组(98.4 克/千克/天);(4) 5X 组(246 克/千克/天)。所有组均连续四周口服营养补充剂。评估包括握力、游泳耐力、力竭测试和血清生化分析。此外,研究还通过基质辅助激光解吸/电离质谱法(MALDI-TOF MS)检测了生物活性肽,并进行了细菌反向突变和急性口服毒性试验,以评估其安全性。研究结果表明,补充 Chlorich®EnergyBoost 后,血清乳酸水平显著降低 14.08% 至 22.54%,血尿素氮水平显著降低 12.23% 至 16.76%,乳酸清除率提高 0.28 至 0.35,肌糖原储存量提高 1.10 至 1.44 倍,肝糖原储存量提高 1.41 至 1.47 倍。这些结果显示了剂量依赖性效应。MALDI-TOF 分析显示了二氢脂酰胺脱氢酶和超氧化物歧化酶的表达。细菌反向突变和急性口服毒性试验均未显示出不良影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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