Antifungal susceptibility pattern of Candida auris strains: Analysis of clinical strains in a tertiary-care educational university hospital.

IF 1.5 4区医学 Q4 MICROBIOLOGY

New Microbiologica Pub Date : 2024-07-01

Amira Khairy, Suha Hejres, Umran Elbahr, Farouq Dayyab, Clark Steven Delos Reyes, Jennie Ann Pastrana, Chithra Vineeth, Shruti Prem Sudha, Ozge Keskin, Shiv Singh Rana, Elias Fadel, Hakan Erdem, Oguz Resat Sipahi

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Abstract

Herein, we aimed to investigate the antifungal susceptibility pattern of Candida auris clinical strains in our setting Bahrain Oncology Center-King Hamad University Hospital-Bahrain. C. auris strains isolated from different clinical specimens in the Microbiology Laboratory from October-2021 to November-2022 were evaluated. Species-level identification of fungi was performed by MALDI-TOF (Bruker, Germany). Minimum inhibitory concentration (MIC) was determined either by E-test strips or by MICRONAUT MIC system based on CDC guidelines for C. auris antifungal interpretation. Fluconazole, amphotericin-B, voriconazole, and caspofungin susceptibility data of the clinical strains were analyzed. A total of 40 clinical isolates were included: 25% were blood culture isolates, 65% were urinary, and 10% were soft tissue isolates. Only 29 strains could be tested for amphotericin-B and 32 for voriconazole. Overall resistance pattern was as follows: 100% resistance to fluconazole, 2.5% resistance to caspofungin, and 0% resistance to amphotericin b. Median voriconazole MIC was 0.015 ug/ml (min 0.08, max= 0.064 ug/ml). We had no fluconazole-sensitive strain and only one caspofungin-resistant strain. A single isolate (2.5%), which was associated with candidemia, demonstrated resistance to two antifungal agents: fluconazole and caspofungin. No triple or quadruple drug resistant strain existed.

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念珠菌菌株的抗真菌药敏模式：分析一家三级医疗大学医院的临床菌株。

在此，我们旨在调查巴林肿瘤中心-哈马德国王大学医院（Bahrain Oncology Center-King Hamad University Hospital-Bahrain）临床念珠菌菌株的抗真菌敏感性模式。我们对 2021 年 10 月至 2022 年 11 月期间微生物实验室从不同临床标本中分离出的念珠菌菌株进行了评估。采用 MALDI-TOF（德国布鲁克公司）对真菌进行了种级鉴定。最小抑菌浓度（MIC）是根据美国疾病预防控制中心（CDC）的抗真菌解释指南，用 E 测试条或 MICRONAUT MIC 系统测定的。分析了临床菌株对氟康唑、两性霉素-B、伏立康唑和卡泊芬净的药敏数据。共纳入 40 株临床分离株：25%为血液培养分离株，65%为尿液分离株，10%为软组织分离株。只有 29 株可进行两性霉素-B 测试，32 株可进行伏立康唑测试。总体耐药性模式如下伏立康唑 MIC 中位数为 0.015 微克/毫升（最低 0.08 微克/毫升，最高 0.064 微克/毫升）。我们没有发现对氟康唑敏感的菌株，只有一株对卡泊芬净耐药的菌株。一个与念珠菌血症相关的分离株（2.5%）显示出对两种抗真菌药物的耐药性：氟康唑和卡泊芬净。不存在三重或四重耐药菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Microbiologica 生物-微生物学

CiteScore

2.20

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.