The particularities of connective fibers from the wall of varicose veins extirpated by cryostripping.

IF 1.2 4区 医学 Q4 DEVELOPMENTAL BIOLOGY
Andrei Florin Părău, Amalia Raluca Ceauşu, Nela Puşa Gaje, Sorin Olariu, Marius Raica
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引用次数: 0

Abstract

Introduction: The varicose vein affects more than 30% of the general population. Significantly increased rates were noticed in women and older population. From the histopathological point of view, venous arterialization, smooth muscle cell hypertrophy, and hyperplasia are the main changes noticed in varicose vein disease. Some of the main therapeutic methods used in the management of varicose disease are injection sclerotherapy, conservative, surgical, saphenous vein inversion and removal, high saphenous ligation, ambulatory phlebectomy, transilluminated powered phlebectomy, endovascular management, cryostripping.

Aim: The aim of this study was to evaluate the morphology of connective fibers from the wall of the varicose veins extirpated by cryostripping.

Patients, materials and methods: The study included 109 samples taken by cryostripping method. Hematoxylin-Eosin, Masson's trichrome, Silver and Orcein staining were applied. The assessment of fibers was made according to score values between 0 and 3.

Results: It was found no major structural differences in terms of alterations of collagen fibers induced by the applied surgical procedure. It was noticed duplications and multiplications of the internal elastic lamina, as diffuse and nodular forms. Depletion of elastic fibers at the media was a lesion identified in most of the specimens. The depletion of reticulin fibers correlates with the accumulation of collagen fibers, which partially or completely replace the network in the media and intima. No correlation was found between changes in the reticulin network and the time between prelevation and buffered formalin fixation, the maximum time investigated being five days.

Conclusions: The Orcein staining in the venous vessel evaluation panel may be a useful investigation.

用冷冻切片法切除静脉曲张壁结缔纤维的特殊性。
简介超过 30% 的普通人群患有静脉曲张。女性和老年人的发病率显著增加。从组织病理学的角度来看,静脉动脉化、平滑肌细胞肥大和增生是静脉曲张疾病的主要变化。治疗静脉曲张疾病的主要方法有注射硬化剂疗法、保守疗法、手术疗法、大隐静脉倒置和切除术、大隐静脉高位结扎术、非卧床静脉切除术、透光动力静脉切除术、血管内治疗、冷冻切削术等:本研究包括109个通过冷冻切片法提取的样本。研究采用了苏木精-伊红、马森三色染色法、银染色法和奥氏染色法。根据 0 至 3 之间的分值对纤维进行评估:结果:结果表明,手术方法引起的胶原纤维改变在结构上没有重大差异。结果发现,内部弹力层呈弥漫和结节状重复和增殖。在大多数标本中都发现了介质弹性纤维耗竭的病变。网状纤维的耗竭与胶原纤维的堆积有关,胶原纤维部分或完全取代了中层和内膜的网络。网状纤维网的变化与预处理和缓冲福尔马林固定之间的时间没有相关性,调查的最长时间为五天:静脉血管评估小组中的奥氏染色可能是一项有用的研究。
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来源期刊
CiteScore
1.70
自引率
20.00%
发文量
221
审稿时长
3-8 weeks
期刊介绍: Romanian Journal of Morphology and Embryology (Rom J Morphol Embryol) publishes studies on all aspects of normal morphology and human comparative and experimental pathology. The Journal accepts only researches that utilize modern investigation methods (studies of anatomy, pathology, cytopathology, immunohistochemistry, histochemistry, immunology, morphometry, molecular and cellular biology, electronic microscopy, etc.).
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