Effect of plasma-activated water on planktonic and biofilm cells of Vibrio parahaemolyticus strains isolated from cutting board surfaces in retail seafood markets.

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mehdi Zarei, Maryam Ghaderi Ghahfarokhi, Mohammad Sabaeian, Mahtab Sepahi, Soraya Alirezaie, Mohadeseh Mohebi
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引用次数: 0

Abstract

Aims: This research aimed to analyze cutting board surfaces in seafood markets to find Vibrio parahaemolyticus, assess the isolates' ability to form biofilms, generate and evaluate characteristics of plasma-activated water (PAW), and compare the effect of PAW on planktonic and biofilm cells of the isolated V. parahaemolyticus strains.

Methods and results: A total of 11 V. parahaemolyticus strains were isolated from 8.87% of the examined cutting boards. Biofilm-forming ability was evaluated for these isolates at temperatures of 10°C, 20°C, and 30°C using crystal violet staining. Four strains with the highest biofilm potential were selected for further analysis. The pH of the PAW used in the study was 3.41 ± 0.04, and the initial concentrations of hydrogen peroxide, nitrate, and nitrite were 108 ± 9.6, 742 ± 61, and 36.3 ± 2.9 µM, respectively. However, these concentrations decreased significantly within 3-4 days during storage at room temperature. PAW exhibited significant antimicrobial effects on V. parahaemolyticus planktonic cells, reducing viable bacteria up to 4.54 log CFU/ml within 20 min. PAW also reduced the number of biofilm cells on stainless steel (up to 3.55 log CFU/cm2) and high-density polyethylene (up to 3.06 log CFU/cm2) surfaces, although to a lesser extent than planktonic cells.

Conclusions: PAW exhibited significant antibacterial activity against V. parahaemolyticus cells, although its antibacterial properties diminished over time. Furthermore, the antibacterial activity of PAW against biofilm cells of V. parahaemolyticus was less pronounced compared to the planktonic cells. Therefore, the actual effectiveness of PAW in seafood processing environments can be affected by biofilms that may form on various surfaces such as cutting boards if they are not cleaned properly.

等离子活化水对从零售海鲜市场砧板表面分离出的副溶血性弧菌浮游细胞和生物膜细胞的影响。
目的:本研究旨在分析海鲜市场中砧板表面的副溶血性弧菌,评估分离菌株形成生物膜的能力,生成并评估等离子活化水(PAW)的特性,比较等离子活化水对分离出的副溶血性弧菌菌株的浮游细胞和生物膜细胞的影响:从 8.87% 的砧板上共分离出 11 株副溶血性弧菌。使用水晶紫染色法评估了这些分离菌株在 10、20 和 30°C 温度下形成生物膜的能力。筛选出生物膜形成能力最强的四种菌株进行进一步分析。研究中使用的 PAW 的 pH 值为 3.41±0.04,过氧化氢、硝酸盐和亚硝酸盐的初始浓度分别为 108±9.6µM、742±61 µM 和 36.3±2.9 µM。不过,在室温下储存 3-4 天后,这些浓度会明显降低。PAW 对副溶血性弧菌浮游细胞有明显的抗菌作用,在 20 分钟内可将存活细菌减少到 4.54 log CFU/ml。PAW 还能减少不锈钢(高达 3.55 log CFU/cm2)和高密度聚乙烯(高达 3.06 log CFU/cm2)表面的生物膜细胞数量,但减少的程度低于浮游细胞:结论:PAW 对副溶血性弧菌细胞具有明显的抗菌活性,但其抗菌特性会随着时间的推移而减弱。此外,与浮游细胞相比,PAW 对副溶血性弧菌生物膜细胞的抗菌活性并不明显。因此,在海鲜加工环境中,如果砧板等各种表面清洁不当,可能会形成生物膜,从而影响 PAW 的实际效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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