Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection.

Abstract

We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor, which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot's (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analyses.