Investigating potential mechanisms of vitamin D against thyroid cancer via network pharmacology and experimental validation

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Bin Liu, Bowen Hou, Yu Zhao, Fengyi Gao, Xiaoyin Dong, Jiageng He
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引用次数: 0

Abstract

Thyroid cancer (TC) is one of the most common endocrine malignancies worldwide. Increasing evidence suggests that vitamin D (VD) has potential benefits in the treatment of TC. However, evidence regarding the targets and molecular mechanisms of VD in TC remains limited. In this study, we conducted network pharmacology, molecular docking, and experimental evaluation to explore the target genes, biological functions, and signaling pathways involved in this process. Network analysis revealed 77 potential target genes of VD against TC, and four hub target genes were identified: ESR1, KIT, CCND1, and PGR. Furthermore, we identified the biological processes (BP) and signaling pathways involving these potential target genes, and then determined the possible interaction between the hub targets and VD through molecular docking. Finally, through in vitro experiments, we found that VD effectively inhibits the proliferation of TC cells and downregulates the expression of the ESR1 gene. In conclusion, the effects of VD against TC involve multiple biological targets, BP, and signaling pathways. These findings provide scientific evidence for the application of VD in the treatment of TC.

Abstract Image

Abstract Image

通过网络药理学和实验验证研究维生素 D 防治甲状腺癌的潜在机制。
甲状腺癌(TC)是全球最常见的内分泌恶性肿瘤之一。越来越多的证据表明,维生素 D(VD)对治疗甲状腺癌有潜在的益处。然而,有关维生素D在甲状腺癌中的靶点和分子机制的证据仍然有限。在本研究中,我们通过网络药理学、分子对接和实验评估来探索这一过程中涉及的靶基因、生物功能和信号通路。网络分析发现了77个VD抗肿瘤的潜在靶基因,并确定了4个中心靶基因:我们发现了四个中心靶基因:ESR1、KIT、CCND1和PGR。此外,我们还确定了涉及这些潜在靶基因的生物过程(BP)和信号通路,然后通过分子对接确定了中心靶基因与 VD 之间可能的相互作用。最后,通过体外实验,我们发现 VD 能有效抑制 TC 细胞的增殖并下调 ESR1 基因的表达。总之,VD 对 TC 的作用涉及多个生物靶点、BP 和信号通路。这些发现为 VD 在 TC 治疗中的应用提供了科学依据。
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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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