Croton grewioides essential oil and anethole reduce oxidative stress and improve growth of bovine primordial follicles during culture of ovarian tissue.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2024-07-17 DOI:10.1093/jpp/rgae093

Felipe F da Silva, Francisco das Chagas Costa, Venância A N Azevedo, Ernando I T de Assis, Geovany A Gomes, Valdevane R Araújo, Selene M de Morais, Tigressa H S Rodrigues, José R V Silva

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引用次数: 0

Abstract

Objectives: This study aims to evaluate the effects of Croton grewioides essential oil (CGEO) and anethole on follicle survival, growth, and oxidative stress in cultured bovine ovarian tissues.

Methods: Ovarian tissues were cultured for 6 days in a medium supplemented with different concentrations (1, 10, 100, or 1000 µg mL-1) of CGEO or anethole and then, follicular survival and growth, collagen content, and stromal cell density in ovarian tissues cultured in vitro were evaluated by histology. The mRNA levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase 1 (GPX1), peroxirredoxin 6 (PRDX6), and nuclear factor erythroid 2-related factor 2 (NRF2) were evaluated by real-time PCR. The activity of SOD, CAT, glutathione peroxidase (GPx), and thiol concentrations were investigated.

Key findings: Ovarian tissues cultured with 1 µg mL-1 CGEO or anethole had a higher percentage of healthy follicles than those cultured in a control medium (P < .05). The 1 µg mL-1 CGEO also increased the number of stromal cells, collagen fibers, and thiol levels. Anethole (1 µg mL-1) increased CAT activity and reduced that of GPx. The activity of SOD was reduced by CGEO. In contrast, 1 µg mL-1 anethole reduced mRNA for CAT, PRDX1, and NRF2 (P < .05). In addition, 1 µg mL-1 CGEO reduced mRNA for CAT, PRDX6, and GPx1 (P < .05).

Conclusions: The presence of 1 µg mL-1 anethole or CGEO in a culture medium promotes follicle survival and regulates oxidative stress and the expression of mRNA and activity of antioxidant enzymes in cultured bovine ovarian tissues.

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巴豆精油和茴香醚在卵巢组织培养过程中可降低氧化应激，改善牛原始卵泡的生长。

研究目的本研究旨在评估巴豆精油（CGEO）和茴香硫醚对培养牛卵巢组织中卵泡存活、生长和氧化应激的影响：在添加不同浓度（1、10、100 或 1000 µg mL-1）CGEO 或茴香硫醚的培养基中培养卵巢组织 6 天，然后用组织学方法评估体外培养卵巢组织中卵泡的存活和生长、胶原蛋白含量和基质细胞密度。实时 PCR 评估了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶 1（GPX1）、过氧化还原酶 6（PRDX6）和核因子红细胞 2 相关因子 2（NRF2）的 mRNA 水平。研究还调查了 SOD、CAT、谷胱甘肽过氧化物酶（GPx）的活性和硫醇浓度：主要发现：与在对照培养基中培养的卵巢组织相比，用 1 µg mL-1 CGEO 或茴香醚培养的卵巢组织中健康卵泡的比例更高（P < .05）。1 µg mL-1 CGEO 还能增加基质细胞的数量、胶原纤维和硫醇水平。茴香醚（1 µg mL-1）提高了 CAT 的活性，降低了 GPx 的活性。CGEO 会降低 SOD 的活性。相反，1 µg mL-1 的茴香醚会降低 CAT、PRDX1 和 NRF2 的 mRNA（P < .05）。此外，1 µg mL-1 CGEO 会降低 CAT、PRDX6 和 GPx1 的 mRNA（P < .05）：结论：在培养基中加入 1 µg mL-1 的茴香醚或 CGEO 可促进卵泡存活，调节氧化应激以及培养牛卵巢组织中抗氧化酶 mRNA 的表达和活性。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.