First record and description of actinospore stages (raabeia, triactinomyxon, and aurantiactinomyxon types) of fish parasitic myxozoans from Malaysia

Abstract

During a 2-month survey in 2023 at Tasik Telabak, Terengganu, Malaysia three distinct actinospore types, namely raabeia, triactinomyxon and aurantiactinomyxon were identified in three invertebrate host species: Aulodrilus acutus, Branchiodrilus sp., and Bothrioneurum sp. utilizing morphometric and molecular analyses. Maximum likelihood of 18S rDNA positioned the raabeia type within the Myxobolus clade from fish of the Order Cypriniformes, suggesting a detected actinospore has a potential life cycle development in Cypriniformes and the genus Myxobolus. Both triactinomyxon and aurantiactinomyxon types were described solely based on morphology and morphometrics due to preservation error preventing the acquisition of 18S rDNA sequences. The triactinomyxon type in this study exhibited distinct morphology in spore shape and dimensions, characterized by a short style and caudal processes. Conversely, the aurantiactinomyxon type described herein possesses prominent elongated pyriform polar capsules not resembling any previously known aurantiactinomyxon types. These distinctive features, along with host species and geographical location justify their classification as novel types. Histological and microscopic analyses revealed the development of pansporocysts in the intestinal epithelium of the oligochaete host. This study marks the first descriptions of actinospore stages of myxozoans in Malaysia and the initial report of actinospores infecting host species of Aulodrilus acutus, Branchiodrilus sp. and Bothrioneurum sp.