Fission yeast Pib2 localizes to vacuolar membranes and regulates TOR complex 1 through evolutionarily conserved domains.

IF 3.5 4区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
Yuichi Morozumi, Yumi Hayashi, Cuong Minh Chu, Fajar Sofyantoro, Yutaka Akikusa, Tomoyuki Fukuda, Kazuhiro Shiozaki
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引用次数: 0

Abstract

TOR complex 1 (TORC1) is a multi-protein kinase complex that coordinates cellular growth with environmental cues. Recent studies have identified Pib2 as a critical activator of TORC1 in budding yeast. Here, we show that loss of Pib2 causes severe growth defects in fission yeast cells, particularly when basal TORC1 activity is diminished by hypomorphic mutations in tor2, the gene encoding the catalytic subunit of TORC1. Consistently, TORC1 activity is significantly compromised in the tor2 hypomorphic mutants lacking Pib2. Moreover, as in budding yeast, fission yeast Pib2 localizes to vacuolar membranes via its FYVE domain, with its tail motif indispensable for TORC1 activation. These results strongly suggest that Pib2-mediated positive regulation of TORC1 is evolutionarily conserved between the two yeast species.

裂殖酵母 Pib2 定位于液泡膜,通过进化保守的结构域调控 TOR 复合物 1。
TOR 复合物 1(TORC1)是一种多蛋白激酶复合物,能根据环境线索协调细胞生长。最近的研究发现,Pib2 是芽殖酵母中 TORC1 的关键激活剂。在这里,我们发现 Pib2 的缺失会导致裂殖酵母细胞出现严重的生长缺陷,尤其是当 TORC1 催化亚基的编码基因 tor2 发生低位突变而导致基础 TORC1 活性降低时。同样,在缺乏 Pib2 的 tor2 基因低倍突变体中,TORC1 的活性也会明显减弱。此外,与芽殖酵母一样,裂殖酵母 Pib2 通过其 FYVE 结构域定位到液泡膜上,其尾部基团对 TORC1 的激活不可或缺。这些结果有力地表明,Pib2 介导的 TORC1 正调控在两种酵母之间是进化保守的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
7.00
自引率
2.90%
发文量
303
审稿时长
1.0 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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