Optimal conditions, experimentation and drug testing

IF 5.9 3区 农林科学 Q1 VETERINARY SCIENCES
Hippokratis Kiaris
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引用次数: 0
最佳条件、实验和药物测试
最佳条件是生物学实验研究的黄金标准。然而,"最佳 "的定义相当模糊,自然界中也不存在最佳条件,而是在次佳条件下运行。这一明显的悖论既影响到以好奇心为驱动力的研究,即寻找基本生物学问题的答案,也影响到临床前研究,即为现实生活中的病症和病人确定药物。例如,培养细胞的温度保持在 37 °C,葡萄糖浓度约为或略高于 5.5 mM,生长因子和营养物质充足。同样,小鼠实验也是将同性动物关在笼子里一起进行的,经常是五只一组。光周期在整个实验过程中保持稳定,通常在 12 小时光照和 12 小时黑暗之间交替进行,温度约为 25 °C,水量充足,在整个研究过程中自由提供食物,并根据特定动物物种选择最佳食物。根据实验的具体目标,如果对这些条件中的任何一个进行研究,都会有系统地改变这些条件,但实验装置保持不变。例如,我们可以测试低温或不同饮食的影响,但其余条件同样保持稳定,重要的是保持最佳状态,这被认为是得出有意义结论的先决条件。
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来源期刊
Lab Animal
Lab Animal 农林科学-兽医学
CiteScore
0.60
自引率
2.90%
发文量
181
审稿时长
>36 weeks
期刊介绍: LabAnimal is a Nature Research journal dedicated to in vivo science and technology that improves our basic understanding and use of model organisms of human health and disease. In addition to basic research, methods and technologies, LabAnimal also covers important news, business and regulatory matters that impact the development and application of model organisms for preclinical research. LabAnimal's focus is on innovative in vivo methods, research and technology covering a wide range of model organisms. Our broad scope ensures that the work we publish reaches the widest possible audience. LabAnimal provides a rigorous and fair peer review of manuscripts, high standards for copyediting and production, and efficient publication.
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