Skoochies and its component substances induced testicular damage and impaired sperm function via increased generation of reactive oxygen species and impairment of the glutathione system in rats

Ayodeji Folorunsho Ajayi Ph.D. , Olufemi Ogundeji Ogundipe M.Tech. , Moses Agbomhere Hamed B.M.L.S. , David Tolulope Oluwole M.Phil.
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引用次数: 0

Abstract

Objective

To examine the effect of skoochies, an illicit cocktail drink, on testicular and sperm function in male rats.

Design

Twenty-five adult male Wistar rats were assigned randomly into five groups (n = 5) as follows: normal saline; skoochies; Cannabis sativa; codeine; and tramadol. The cocktail (skoochies) used in this study was formulated with the following composition: codeine (5 mg/kg); tramadol (20 mg/kg); and cannabis extract (2 mg/kg). These doses are as previously reported. Administration was performed once daily for 28 days.

Setting

University.

Animal(s)

Twenty-five (25) male Wistar rats.

Intervention(s)

Skoochies, tramadol, Codeiene, Cannabis.

Main Outcome Measure(s)

Skoochies and its components induced testicular and sperm damage via increased generation of reactive oxygen species and impairment of glutathione system in rats.

Result(s)

Skoochies increased reactive oxygen species generation and impaired the antioxidant system resulting in inflammation that eventually damaged the testicular tissue. Skoochies caused oxidoinflammatory injury to this tissue, leading to impaired testicular function. This was evident by the distorted cytoarchitecture, reduced sperm count and motility, and impaired testicular deoxyribonucleic acid integrity.

Conclusion(s)

Thus, our results infer that skoochies impaired the testicular and sperm function through the increased generation of reactive oxygen species and impairment of the glutathione system.
Skoochies 及其成分物质通过增加活性氧的生成和损害谷胱甘肽系统,导致大鼠睾丸损伤和精子功能受损。
研究目的研究非法鸡尾酒饮料 Skoochies 对雄性大鼠睾丸和精子功能的影响:将 25 只成年雄性 Wistar 大鼠随机分为以下 5 组(n = 5):正常生理盐水(NS 组)、skoochies(SK 组)、大麻(CS 组)、可待因(CO 组)和曲马多(TM 组)。本研究使用的鸡尾酒(skoochies)由以下成分配制而成:可待因(5 毫克/千克)、曲马多(20 毫克/千克)和大麻提取物(2 毫克/千克)。这些剂量与之前报告的剂量相同。每天给药一次,持续二十八(28)天:结果:Skoochies 增加了活性氧的生成,损害了抗氧化系统,导致炎症,最终损害了睾丸组织。Skoochies 对睾丸组织造成氧化-炎症损伤,导致睾丸功能受损。这表现在细胞结构扭曲、精子数量减少、活力降低以及睾丸 DNA 完整性受损等方面:因此,我们的研究结果推断,Skoochies 通过增加活性氧的生成和损害谷胱甘肽系统,导致睾丸和精子功能受损。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
F&S science
F&S science Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
51 days
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