[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].

Q4 Medicine

上海口腔医学 Pub Date : 2024-04-01

Ying Yu, Qing-Qing Weng, Yin-Yue Luo, Shu-Ran Yao, Fang-Yu Yi, Ying Zhang

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引用次数: 0

Abstract

Purpose: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.

Methods: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.

Results: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.

Conclusions: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.

本刊更多论文

[氟中毒小鼠髁突软骨自噬相关因子 LC3 和 p62 的损伤及表达变化]。

目的：研究氟中毒小鼠髁突软骨的损伤及LC3和p62的表达：将 30 只 4 周龄雄性 C57BL/6 小鼠随机分为对照组和实验组，每组 15 只。对照组饮用普通饮用水，实验组饮用含氟量为 75 mg/L 的饮用水，连续 8 周。髁状突软骨的结构通过改良的黄绿素 O-快绿FCF软骨染色试剂盒进行观察。免疫组化法检测MMP-13、Ⅱ型胶原蛋白、LC3和p62的表达。使用 SPSS 22.0 软件包对免疫组化的半定量结果进行双向方差分析：免疫组化结果显示，实验组 MMP-13 和 LC3 表达增加，Ⅱ型胶原和 p62 表达减少：结论：饮用含氟量为 75 毫克/升的水会导致小鼠髁突软骨退化和自噬。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

上海口腔医学 Medicine-Medicine (all)

CiteScore

0.30

自引率

0.00%

发文量

5299

期刊介绍：