[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].

Q4 Medicine

上海口腔医学 Pub Date : 2024-04-01

Ying Yu, Qing-Qing Weng, Yin-Yue Luo, Shu-Ran Yao, Fang-Yu Yi, Ying Zhang

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引用次数: 0

Abstract

Purpose: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.

Methods: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.

Results: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.

Conclusions: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.

本刊更多论文

[氟中毒小鼠髁突软骨自噬相关因子 LC3 和 p62 的损伤及表达变化]。

目的：研究氟中毒小鼠髁突软骨的损伤及LC3和p62的表达：将 30 只 4 周龄雄性 C57BL/6 小鼠随机分为对照组和实验组，每组 15 只。对照组饮用普通饮用水，实验组饮用含氟量为 75 mg/L 的饮用水，连续 8 周。髁状突软骨的结构通过改良的黄绿素 O-快绿FCF软骨染色试剂盒进行观察。免疫组化法检测MMP-13、Ⅱ型胶原蛋白、LC3和p62的表达。使用 SPSS 22.0 软件包对免疫组化的半定量结果进行双向方差分析：免疫组化结果显示，实验组 MMP-13 和 LC3 表达增加，Ⅱ型胶原和 p62 表达减少：结论：饮用含氟量为 75 毫克/升的水会导致小鼠髁突软骨退化和自噬。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

上海口腔医学 Medicine-Medicine (all)

CiteScore

0.30

自引率

0.00%

发文量

5299

期刊介绍： "Shanghai Journal of Stomatology (SJS)" is a comprehensive academic journal of stomatology directed by Shanghai Jiao Tong University and sponsored by the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. The main columns include basic research, clinical research, column articles, clinical summaries, reviews, academic lectures, etc., which are suitable for reference by clinicians, scientific researchers and teaching personnel at all levels engaged in oral medicine.