Metagenomic Analysis of Rhizospheric Bacterial Community of Citrus Trees Expressing Phloem-Directed Antimicrobials.

IF 3.3 3区 生物学 Q2 ECOLOGY
Leandro Alberto Núñez-Muñoz, Martín Eduardo Sánchez-García, Berenice Calderón-Pérez, Rodolfo De la Torre-Almaraz, Roberto Ruiz-Medrano, Beatriz Xoconostle-Cázares
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Abstract

Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tissues. This study investigated the effects of genetically modified citrus trees expressing Citrus sinensis PP16 (CsPP16) fused to human lysozyme and β-defensin-2 on the soil microbiome diversity using 16S amplicon analysis. The results indicated that there were no significant alterations in alpha diversity, beta diversity, phylogenetic diversity, differential abundance, or functional prediction between the antimicrobial phloem-overexpressing plants and the control group, suggesting minimal impact on microbial community structure. However, microbiota diversity analysis revealed distinct bacterial assemblages between the rhizosphere soil and root environments. This study helps to understand the ecological implications of crops expressing phloem-targeted antimicrobials for vascular disease management, with minimal impact on soil microbiota.

Abstract Image

表达韧皮部定向抗菌素的柑橘树根瘤菌群落的元基因组分析
黄龙病又称柑橘绿化病,是目前最具破坏性的柑橘病害,但预防和缓解效果有限。一种很有前景的黄龙病防治策略是将抗菌剂与针对维管组织的载体蛋白(16 kDa 的韧皮部蛋白或 PP16)融合在一起。本研究利用 16S 扩增片段分析法研究了表达融合了人溶菌酶和 β防御素-2 的柑橘 PP16(CsPP16)的转基因柑橘树对土壤微生物组多样性的影响。结果表明,抗菌噬菌体高表达植物与对照组之间在α多样性、β多样性、系统发育多样性、丰度差异或功能预测方面没有明显变化,表明对微生物群落结构的影响极小。不过,微生物群多样性分析显示,根圈土壤和根部环境中的细菌群落截然不同。这项研究有助于了解作物表达韧皮部靶向抗菌剂对维管束病害管理的生态影响,同时对土壤微生物群的影响极小。
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来源期刊
Microbial Ecology
Microbial Ecology 生物-海洋与淡水生物学
CiteScore
6.90
自引率
2.80%
发文量
212
审稿时长
3-8 weeks
期刊介绍: The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.
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