Immunogenicity of mannan derived from Mycobacterium bovis as a promising adjuvant in vaccine BCG.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-06-01 DOI:10.18502/ijm.v16i3.15767

Rajab Mardani, Ariana Alavi, Seyed Dawood Mousavi Nasab, Nayebali Ahmadi, Mohammad Javad Hossein Tehrani, Maryam Shahali, Delaram Doroud

{"title":"Immunogenicity of mannan derived from Mycobacterium bovis as a promising adjuvant in vaccine BCG.","authors":"Rajab Mardani, Ariana Alavi, Seyed Dawood Mousavi Nasab, Nayebali Ahmadi, Mohammad Javad Hossein Tehrani, Maryam Shahali, Delaram Doroud","doi":"10.18502/ijm.v16i3.15767","DOIUrl":null,"url":null,"abstract":"Background and objectives: Lipoarabinomannan is one of the components of the significant structural cell surfaces of mycobacteria and serves as an immunostimulatory factor. TNF-α and IL-12 are two examples of the anti-bacterial inflammatory cytokines that are activated and induced during infection.Materials and methods: In this study, mannan was extracted and processed, and then Bulb/c female mice were used in three groups, one group was given BCG vaccine, the other group was given BCG vaccine with mannan adjuvant, and a non-injected group was used as a control group. Inflammatory factors interleukin-12, TNF-α, IgG and IgM were measured in mouse serum.Results: The levels of the inflammatory factors interleukin-12 and TNF-α in the serum isolated from mice receiving the BCG vaccine with mannan adjuvant showed a significant difference compared to the group that received only the BCG vaccine and the control group [IL-12] and , with P≤0.05.The examination of the level of IgG immune factors in these three groups revealed a significant difference. The group that received the BCG vaccine with mannan adjuvant showed a marked contrast compared to the group that received only the BCG vaccine and the control group, with P≤0.05. The level of IgM was higher in the group that received the BCG vaccine alone compared to the adjuvant vaccine group and the control group, with P≤0.05.Conclusion: Our results indicated that mice receiving the BCG vaccine with mannan adjuvant had significantly higher serum levels of IL-12, TNF-α, and IgG than the group receiving BCG alone.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245356/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i3.15767","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and objectives: Lipoarabinomannan is one of the components of the significant structural cell surfaces of mycobacteria and serves as an immunostimulatory factor. TNF-α and IL-12 are two examples of the anti-bacterial inflammatory cytokines that are activated and induced during infection.

Materials and methods: In this study, mannan was extracted and processed, and then Bulb/c female mice were used in three groups, one group was given BCG vaccine, the other group was given BCG vaccine with mannan adjuvant, and a non-injected group was used as a control group. Inflammatory factors interleukin-12, TNF-α, IgG and IgM were measured in mouse serum.

Results: The levels of the inflammatory factors interleukin-12 and TNF-α in the serum isolated from mice receiving the BCG vaccine with mannan adjuvant showed a significant difference compared to the group that received only the BCG vaccine and the control group [IL-12] and , with P≤0.05.The examination of the level of IgG immune factors in these three groups revealed a significant difference. The group that received the BCG vaccine with mannan adjuvant showed a marked contrast compared to the group that received only the BCG vaccine and the control group, with P≤0.05. The level of IgM was higher in the group that received the BCG vaccine alone compared to the adjuvant vaccine group and the control group, with P≤0.05.

Conclusion: Our results indicated that mice receiving the BCG vaccine with mannan adjuvant had significantly higher serum levels of IL-12, TNF-α, and IgG than the group receiving BCG alone.

查看原文本刊更多论文

从牛分枝杆菌中提取的甘露聚糖作为卡介苗佐剂的免疫原性。

背景和目的：脂质抗原甘露聚糖是分枝杆菌重要的细胞表面结构成分之一，是一种免疫刺激因子。TNF-α和IL-12是感染过程中激活和诱导的两种抗菌炎症细胞因子：本研究对甘露聚糖进行提取和处理，然后将 Bulb/c 雌性小鼠分为三组，一组接种卡介苗，另一组接种含甘露聚糖佐剂的卡介苗，未接种组作为对照组。检测小鼠血清中的炎症因子白细胞介素-12、TNF-α、IgG和IgM：从接种卡介苗并添加甘露聚糖佐剂的小鼠血清中分离出的炎症因子白细胞介素-12和TNF-α的水平与只接种卡介苗组和对照组[IL-12]和Ⅴ相比差异显著，P≤0.05。接种含甘露聚糖佐剂卡介苗的组与只接种卡介苗的组和对照组相比差异明显，P≤0.05。单独接种卡介苗组的 IgM 水平高于佐剂疫苗组和对照组，P≤0.05：我们的研究结果表明，接种添加甘露聚糖佐剂的卡介苗的小鼠血清中IL-12、TNF-α和IgG水平明显高于单独接种卡介苗的小鼠。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.