Transcriptome analysis of the degradation process of organic nitrogen by two heterotrophic nitrifying and aerobic denitrifying bacteria.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-07-15 DOI:10.1007/s00203-024-04076-3

Yijun Huang, Xiaoting Liu, Yue Jin, Qichun Zhang

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Abstract

The heterotrophic nitrification aerobic denitrification bacteria (HNDS) can perform nitrification and denitrification at the same time. Two HNDS strains, Achromobacter sp. HNDS-1 and Enterobacter sp. HNDS-6 which exhibited an amazing ability to solution nitrogen (N) removal have been successfully isolated from paddy soil in our lab. When peptone or ammonium sulfate as sole N source, no significant difference in gene expression related to nitrification and denitrification of the strains was found according to the transcriptome analysis. The expression of phosphomethylpyrimidine synthase (thiC), ABC transporter substrate-binding protein, branched-chain amino acid ABC transporter substrate-binding protein, and RNA polymerase (rpoE) in HNDS-1 were significantly upregulated when used peptone as N source, while the expression of exopolysaccharide production protein (yjbE), RNA polymerase (rpoC), glutamate synthase (gltD) and ABC-type branched-chain amino acid transport systems in HNDS-6 were significantly upregulated. This indicated that these two strains are capable of using organic N and converting it into NH₄⁺-N, then utilizing NH₄⁺-N to synthesize amino acids and proteins for their own growth, and strain HNDS-6 can also remove NH₄⁺-N through nitrification and denitrification.

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两种异养硝化细菌和好氧反硝化细菌降解有机氮过程的转录组分析。

异养硝化需氧反硝化细菌（HNDS）可同时进行硝化和反硝化。本实验室从水稻田土壤中成功分离出两株 HNDS 菌株，即 Achromobacter sp.在以蛋白胨或硫酸铵为唯一氮源时，根据转录组分析，发现菌株的硝化和反硝化相关基因的表达没有显著差异。以蛋白胨为氮源时，HNDS-1中的磷甲基嘧啶合成酶（thiC）、ABC转运体底物结合蛋白、支链氨基酸ABC转运体底物结合蛋白和RNA聚合酶（rpoE）的表达显著上调、而在 HNDS-6 中，外多糖生产蛋白（yjbE）、RNA 聚合酶（rpoC）、谷氨酸合成酶（gltD）和 ABC 型支链氨基酸转运系统的表达均显著上调。这表明这两种菌株都能利用有机氮并将其转化为 NH4+-N，然后利用 NH4+-N 合成氨基酸和蛋白质供自身生长，菌株 HNDS-6 还能通过硝化和反硝化去除 NH4+-N。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.