"Immunocytochemistry in Cytology: Myth or Reality": Unraveling the Myth - Immunocytochemistry Applications in Thyroid Lesions.

IF 1.6 4区医学 Q3 PATHOLOGY

Acta Cytologica Pub Date : 2024-07-22 DOI:10.1159/000540366

Mitsuyoshi Hirokawa, Ayana Suzuki

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引用次数: 0

Abstract

Background: Fine-needle aspiration cytology serves as an important preoperative diagnostic tool for thyroid nodules. Despite its excellent diagnostic accuracy, diagnoses based solely on morphological observation can be challenging. Therefore, various ancillary diagnostic techniques have been applied, including immunocytochemistry (ICC). This review discusses the application and evaluation of ICC in thyroid fine needle aspiration.

Summary: Currently, three immunostaining preparation methods are available for cytological materials: liquid-based cytology, cell block, and cell transfer. ICC proves valuable in scenarios such as tumour diagnosis, assessment of differentiation and grading of carcinomas, estimation of primary organs in metastatic carcinomas, and detection of gene abnormalities. However, ICC, while useful, is not as accurate as immunohistochemistry and is more difficult to evaluate.

Key messages: If the pitfalls and limitations are understood and effectively navigated, ICC could play a significant role in decreasing the non-diagnostic rate, thus leading to more accurate and valuable diagnoses and reductions in the re-aspiration rate.

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揭开神话：免疫细胞化学在甲状腺病变中的应用。

背景：细针穿刺细胞学是甲状腺结节的重要术前诊断工具。尽管细针穿刺细胞学诊断准确性极高，但仅凭形态学观察进行诊断仍具有挑战性。因此，各种辅助诊断技术得到了应用，包括免疫细胞化学（ICC）。摘要：目前，细胞学材料有三种免疫染色制备方法：液基细胞学、细胞块和细胞转移。ICC 在肿瘤诊断、评估癌的分化和分级、估计转移癌的原发器官以及检测基因异常等方面具有重要价值。不过，ICC 虽然有用，但不如免疫组化准确，而且更难评估：关键信息：如果能了解并有效利用其中的陷阱和局限性，ICC 将在降低非诊断率方面发挥重要作用，从而获得更准确、更有价值的诊断，并降低再吸入率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Cytologica 生物-病理学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

4-8 weeks

期刊介绍： With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.