The genome history of Bacillus thuringiensis subsp. tenebrionis: From discovery to present day product

IF 1.7 3区农林科学 Q2 ENTOMOLOGY

Journal of Applied Entomology Pub Date : 2024-07-13 DOI:10.1111/jen.13325

Lea Schäfer, Johannes A. Jehle, Regina G. Kleespies, Jörg T. Wennmann

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Abstract

Bacillus thuringiensis subsp. tenebrionis (Btt) was discovered in 1982 and has been used as a biological control agent against the Colorado potato beetle (Leptinotarsa decemlineata) since the early 1990s. In this study, we sequenced, assembled and analysed the genome of strain DSM 2803, which is the patent deposit of the original Btt isolate BI 256‐82. A high‐quality reference genome is provided that allows investigating the genomic changes throughout the commercial history of Btt. Strain DSM 2803 (BI 256‐82) is particularly interesting since the subspecies description of ‘tenebrionis’ is based on it and because it represents the genomic origin of the commercial Btt strain currently used in plant protection. Comparative genome analysis of six Btt strains derived from BI 256‐82 revealed that the genomes were generally highly similar. However, some plasticity in the profile of extrachromosomal elements was detected, including recombination‐dependent plasmid differences and the presence/absence of a circular plasmid‐like prophage.

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苏云金芽孢杆菌亚种的基因组历史：从发现到今天的产品

苏云金芽孢杆菌（Bacillus thuringiensis subsp. tenebrionis，Btt）于 1982 年被发现，自 20 世纪 90 年代初以来一直被用作防治科罗拉多马铃薯甲虫（Leptinotarsa decemlineata）的生物防治剂。在这项研究中，我们对菌株 DSM 2803 的基因组进行了测序、组装和分析，该菌株是原始 Btt 分离物 BI 256-82 的专利保存物。该研究提供了高质量的参考基因组，有助于研究 Btt 在整个商业化历史中的基因组变化。菌株 DSM 2803（BI 256-82）特别有意思，因为 "tenebrionis "亚种的描述就是基于它，而且它代表了目前用于植物保护的商业 Btt 菌株的基因组起源。对源自 BI 256-82 的六个 Btt 菌株进行的基因组比较分析表明，这些菌株的基因组总体上高度相似。然而，在染色体外元素的概况中发现了一些可塑性，包括重组依赖性质粒差异和环状质粒样原生质体的存在/不存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Entomology 生物-昆虫学

CiteScore

3.40

自引率

5.30%

发文量

132

审稿时长

6 months

期刊介绍： The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.