Emanuel Vacalie , Daniel Preda , Petruta Oancea , Anca Ruxandra Leonties , Ludmila Aricov , Adina Raducan
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引用次数: 0
Abstract
Laccases are enzymes with low substrate specificity that in certain cases require the presence of a mediator in order to work properly. Therefore, this paper investigated the degradation of Orange G, Ponceau 4 R and Methyl Red in the presence of laccase isolated from Trametes versicolor and laccase-ABTS system. Laccase, in high concentration, was able to oxidize only Orange G and Ponceau 4 R, with a degree of decolorization less than 10 % in 20 min. In the presence of laccase-ABTS system, the reaction is much faster, and at least 40 % of dyes were degraded in 15 min. A kinetic model accounting for enzymatic oxidation of all dyes, including a step for enzyme operational inactivation, was proposed. This study proves that we can use a single kinetic model for the oxidation of all dyes in the laccase-ABTS system.
期刊介绍：
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.
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