Promiscuous acyltransferases for ester and amide synthesis in aqueous solution

IF 5.2 2区化学 Q1 CHEMISTRY, APPLIED

Catalysis Today Pub Date : 2024-07-09 DOI:10.1016/j.cattod.2024.114925

Benjamin Baumert , Hannes Meinert , Clemens Cziegler , Henrik Terholsen , Thomas Bayer , Uwe T. Bornscheuer

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引用次数: 0

Abstract

Hydrolases are well-known for hydrolyzing esters, amides, carbamates, peptides, or acid anhydrides in the presence of water. However, some of them are also capable of catalyzing the reverse reaction (condensation) under certain conditions in aqueous systems. Hence, these enzymes are called promiscuous hydrolases/acyltransferases. This review deals with their discovery, background information on their mechanism of action, and significant improvements by enzyme engineering to both enhance product formation and decrease the undesired hydrolysis of the targeted acyl products. Their applications in biocatalysis are exemplified by the synthesis of a wide range of esters or amides in aqueous systems, including preparative-scale processes and the combination of hydrolases/acyltransferases with other enzymes in cascade reactions to utilize alternative feedstocks from renewable resources, for example. Complementary, the use of ATP-dependent amide synthesizing enzymes is covered. Together, promiscuous hydrolases/acyltransferases represent practically useful alternatives to well-established chemical reactions, operating in aqueous solutions that also appeal to different industries.

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在水溶液中合成酯和酰胺的杂化酰基转移酶

众所周知，水解酶可以在有水的情况下水解酯、酰胺、氨基甲酸酯、肽或酸酐。不过，其中一些酶在水性体系中的某些条件下也能催化反向反应（缩合）。因此，这些酶被称为杂合水解酶/酰基转移酶。本综述介绍了这些酶的发现、作用机理的背景信息，以及通过酶工程对其进行的重大改进，以增强产品的形成并减少目标酰基产品的意外水解。它们在生物催化中的应用体现在水性体系中各种酯类或酰胺的合成，包括制备规模工艺，以及水解酶/酰基转移酶与其他酶在级联反应中的结合，例如利用可再生资源中的替代原料。此外，还包括使用 ATP 依赖性酰胺合成酶。总之，杂合水解酶/酰基转移酶代表了成熟化学反应的实用替代品，可在水溶液中操作，对不同行业也有吸引力。

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来源期刊

Catalysis Today 化学-工程：化工

CiteScore

11.50

自引率

3.80%

发文量

573

审稿时长

2.9 months

期刊介绍： Catalysis Today focuses on the rapid publication of original invited papers devoted to currently important topics in catalysis and related subjects. The journal only publishes special issues (Proposing a Catalysis Today Special Issue), each of which is supervised by Guest Editors who recruit individual papers and oversee the peer review process. Catalysis Today offers researchers in the field of catalysis in-depth overviews of topical issues. Both fundamental and applied aspects of catalysis are covered. Subjects such as catalysis of immobilized organometallic and biocatalytic systems are welcome. Subjects related to catalysis such as experimental techniques, adsorption, process technology, synthesis, in situ characterization, computational, theoretical modeling, imaging and others are included if there is a clear relationship to catalysis.

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