Expression of dihomo-γ-linolenic acid and FADS1/2 and ELOVL2/5 in term rabbit placentas

IF 3
M. Kyogashima , K. Kamijima , N. Takai , T. Nakajima , T. Mikuma , H. Komamura , K. Asai , M. Ishihara , E. Sugiyama , N. Tanaka
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引用次数: 0

Abstract

Long-chain polyunsaturated fatty acids (LCPUFAs) are essential for both fetal and placental development. We characterized the FA composition and gene expression levels of FA-metabolizing enzymes in rabbit placentas. Total FA compositions from term rabbit placentas (n = 7), livers, and plasma (both n = 4) were examined: among LCPUFAs with more than three double bonds, dihomo-γ-linolenic acid (DGLA) was the most abundant (11.4 ± 0.69 %, mean ± SE), while arachidonic acid was the second-most rich component (6.90 ± 0.56 %). DGLA was barely detectable (<1 %) in livers and plasma from term rabbits, which was significantly lower than in placentas (both p < 0.0001). Compared with the liver, transcript levels of the LCPUFA-metabolizing enzymes FADS2 and ELOVL5 were 7- and 4.5-fold higher in placentas (both p < 0.05), but levels of FADS1 and ELOVL2 were significantly lower (both p < 0.01). Our results suggest a placenta-specific enzyme expression pattern and LCPUFA profile in term rabbits, which may support a healthy pregnancy.

二氢-γ-亚麻酸、FADS1/2 和 ELOVL2/5 在足月兔胎盘中的表达。
长链多不饱和脂肪酸(LCPUFAs)对胎儿和胎盘的发育至关重要。我们研究了兔胎盘中的脂肪酸组成和脂肪酸代谢酶的基因表达水平。我们检测了足月兔胎盘(n = 7)、肝脏和血浆(均为 n = 4)中的总脂肪酸组成:在具有三个以上双键的 LCPUFAs 中,二氢-γ-亚麻酸(DGLA)含量最高(11.4 ± 0.69 %,平均值 ± SE),而花生四烯酸含量次之(6.90 ± 0.56 %)。几乎检测不到 DGLA (
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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
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0
审稿时长
64 days
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