Methodological approaches to studying phase separation and HIV-1 replication: Current and future perspectives

IF 4.2 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2024-07-14 DOI:10.1016/j.ymeth.2024.07.002

Andrew J. Mouland , Bao-An Chau , Vladimir N. Uversky

引用次数: 0

Abstract

This article reviews tried-and-tested methodologies that have been employed in the first studies on phase separating properties of structural, RNA-binding and catalytic proteins of HIV-1. These are described here to stimulate interest for any who may want to initiate similar studies on virus-mediated liquid–liquid phase separation. Such studies serve to better understand the life cycle and pathogenesis of viruses and open the door to new therapeutics.

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研究相分离和 HIV-1 复制的方法：当前和未来展望。

本文回顾了在对 HIV-1 的结构蛋白、RNA 结合蛋白和催化蛋白的相分离特性进行首次研究时所采用的经过试验和测试的方法。本文对这些方法进行了介绍，以激发那些希望对病毒介导的液-液相分离进行类似研究的人的兴趣。这些研究有助于更好地了解病毒的生命周期和致病机理，并为新疗法打开大门。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.