Identification of full-length genes involved in the biosynthesis of β-caryophyllene and lupeol from the leaf transcriptome of Ayapana triplinervis.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2024-11-01 Epub Date: 2024-07-12 DOI:10.1139/gen-2024-0005
Tanuja, Madasamy Parani
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引用次数: 0

Abstract

β-Caryophyllene possesses potential anticancer properties against various cancers, including breast, colon, and lung cancer. Therefore, the essential oil of Ayapana triplinervis, which is rich in β-caryophyllene, can be a potential herbal remedy for treating cancer. However, molecular and genomic studies on A. triplinervis are still sparse. In this study, we obtained 14.7 Gb of RNA-Seq data from A. triplinervis leaf RNA and assembled 137 554 transcripts with an N50 value of 1437 bp. We annotated 72 436 (52.7%) transcripts and mapped 10 640 transcripts to 156 biochemical pathways. Among them, 218 were related to terpenoid backbone biosynthesis, while 27 were linked to sesquiterpenoid and triterpenoid pathways. Ninety-four transcripts were annotated in the β-caryophyllene and lupeol pathways. From these transcripts, for the first time, we identified 25 full-length genes encoding all the 17 enzymes involved in β-caryophyllene biosynthesis and an additional five genes involved in lupeol biosynthesis. These genes will be useful for the metabolic engineering of β-caryophyllene and lupeol biosynthesis, not just in A. triplinervis but also in other species.

从 Ayapana triplinervis 的叶片转录组中鉴定参与β-叶绿素和羽扇豆醇生物合成的全长基因。
β-茶叶烯具有潜在的抗癌特性,可对抗各种癌症,包括乳腺癌、结肠癌和肺癌。因此,富含 β-茶叶烯的 Ayapana triplinervis 精油可作为治疗癌症的潜在草药。然而,关于三叶芹的分子和基因组研究仍然很少。在这项研究中,我们从三叶草叶片 RNA 中获得了 14.7 Gb 的 RNA-Seq 数据,并组装了 1,37,554 个转录本,N50 值为 1,437 bp。我们注释了 72,436 个(52.7%)转录本,并将 10,640 个转录本映射到 156 个生化途径。其中,218 个转录本与萜类骨架的生物合成有关,27 个转录本与倍半萜和三萜类途径有关。94个转录本被注释为β-石竹烯和羽扇豆醇途径。从这些转录本中,我们首次发现了 25 个全长基因,这些基因编码了参与 β-石竹烯生物合成的全部 17 种酶,以及参与羽扇豆醇生物合成的另外 5 个基因。这些基因将有助于β-石竹烯和羽扇豆醇生物合成的新陈代谢工程,不仅适用于A. triplinervis,也适用于其他物种。关键词:β-石竹烯 Eupatorium ayapana Eupatorium triplinervis lupeol 转录组
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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