Trends in co-fractionation mass spectrometry: A new gold-standard in global protein interaction network discovery

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2024-07-11 DOI:10.1016/j.sbi.2024.102880

Raghuveera Kumar Goel , Nazmin Bithi , Andrew Emili

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引用次数: 0

Abstract

Co-fractionation mass spectrometry (CF-MS) uses biochemical fractionation to isolate and characterize macromolecular complexes from cellular lysates without the need for affinity tagging or capture. In recent years, this has emerged as a powerful technique for elucidating global protein-protein interaction networks in a wide variety of biospecimens. This review highlights the latest advancements in CF-MS experimental workflows including machine learning-guided analyses, for uncovering dynamic and high-resolution protein interaction landscapes with enhanced sensitivity, accuracy and throughput, enabling better biophysical characterization of endogenous protein complexes. By addressing challenges and emergent opportunities in the field, this review underscores the transformative potential of CF-MS in advancing our understanding of functional protein interaction networks in health and disease.

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共分馏质谱的发展趋势：发现全球蛋白质相互作用网络的新黄金标准

协同分馏质谱法（CF-MS）利用生化分馏技术从细胞裂解物中分离和鉴定大分子复合物，而无需亲和标记或捕获。近年来，这项技术已成为阐明各种生物样本中全球蛋白质-蛋白质相互作用网络的强大技术。本综述重点介绍了 CF-MS 实验工作流程的最新进展，包括机器学习指导的分析，以提高灵敏度、准确性和通量，揭示动态和高分辨率的蛋白质相互作用图谱，从而更好地对内源性蛋白质复合物进行生物物理表征。通过探讨该领域面临的挑战和新出现的机遇，本综述强调了 CF-MS 在促进我们对健康和疾病中蛋白质功能相互作用网络的了解方面所具有的变革潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation