A systematic study on the effect of protonation and deuteration on electron spin Tm/T2 in a cellular context†

IF 2.9 3区化学 Q3 CHEMISTRY, PHYSICAL

Physical Chemistry Chemical Physics Pub Date : 2024-07-11 DOI:10.1039/D4CP00599F

Francesco Torricella, Valentina Vitali and Lucia Banci

引用次数: 0

Abstract

In recent years, DEER experiments in pulsed EPR have garnered interest for their precise distance distribution insights in cellular and buffer setups. These measurements linked to electron spin T_m/T₂ values of the labelled sample are impacted by the cellular environment being fully protonated or deuterated, as demonstrated in the present study.

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系统研究细胞中质子化和氘化对电子自旋 Tm/T2 的影响

近年来，脉冲 EPR 中的 DEER 实验因其在细胞和缓冲设置中精确的距离分布洞察力而备受关注。这项技术与电子自旋 Tm/T2 有关，标记样品会受到完全质子化或氘化的细胞环境影响，我们的研究就证明了这一点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physical Chemistry Chemical Physics 化学-物理：原子、分子和化学物理

CiteScore

5.50

自引率

9.10%

发文量

2675

审稿时长

2.0 months

期刊介绍： Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.