A prospective study of the proteome of equine pre-implantation embryo.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2024-07-01 DOI:10.1111/rda.14663

Antônio Carlos de A Teles Filho, Deisy J D Sanchez, Arabela G A Viana, Sheheryar Sheheryar, Denise D Guerreiro, Ivan C Bustamante-Filho, Aline M A Martins, Marcelo V Sousa, Carlos A O Ricart, Wagner Fontes, Arlindo A Moura

{"title":"A prospective study of the proteome of equine pre-implantation embryo.","authors":"Antônio Carlos de A Teles Filho, Deisy J D Sanchez, Arabela G A Viana, Sheheryar Sheheryar, Denise D Guerreiro, Ivan C Bustamante-Filho, Aline M A Martins, Marcelo V Sousa, Carlos A O Ricart, Wagner Fontes, Arlindo A Moura","doi":"10.1111/rda.14663","DOIUrl":null,"url":null,"abstract":"<p><p>The present study was conducted to investigate the global proteome of 8-day-old equine blastocysts. Follicular dynamics of eight adult mares were monitored by ultrasonography and inseminated 24 h after the detection of a preovulatory follicle. Four expanded blastocysts were recovered, pooled, and subjected to protein extraction and mass spectrometry. Protein identification was conducted based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, and PepExplorer). Enrichment analysis was performed using g:Profiler, Panther, and String platforms. After the elimination of identification redundancies among search tools (at three levels, based on identifiers, peptides, and cross-database mapping), 1977 proteins were reliably identified in the samples of equine embryos. Proteomic analysis unveiled robust metabolic activity in the 8-day equine embryo, highlighted by an abundance of proteins engaged in key metabolic pathways like the TCA cycle, ATP biosynthesis, and glycolysis. The prevalence of chaperones among highly abundant proteins suggests that regulation of protein folding, and degradation is a key process during embryo development. These findings pave the way for developing new strategies to improve equine embryo media and optimize in vitro fertilization techniques.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction in Domestic Animals","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/rda.14663","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

The present study was conducted to investigate the global proteome of 8-day-old equine blastocysts. Follicular dynamics of eight adult mares were monitored by ultrasonography and inseminated 24 h after the detection of a preovulatory follicle. Four expanded blastocysts were recovered, pooled, and subjected to protein extraction and mass spectrometry. Protein identification was conducted based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, and PepExplorer). Enrichment analysis was performed using g:Profiler, Panther, and String platforms. After the elimination of identification redundancies among search tools (at three levels, based on identifiers, peptides, and cross-database mapping), 1977 proteins were reliably identified in the samples of equine embryos. Proteomic analysis unveiled robust metabolic activity in the 8-day equine embryo, highlighted by an abundance of proteins engaged in key metabolic pathways like the TCA cycle, ATP biosynthesis, and glycolysis. The prevalence of chaperones among highly abundant proteins suggests that regulation of protein folding, and degradation is a key process during embryo development. These findings pave the way for developing new strategies to improve equine embryo media and optimize in vitro fertilization techniques.

查看原文本刊更多论文

马胚胎植入前蛋白质组的前瞻性研究。

本研究旨在调查 8 天大的马胚泡的全蛋白质组。通过超声波检查监测八匹成年母马的卵泡动态，并在检测到排卵前卵泡 24 小时后进行人工授精。回收四个膨大的囊胚，汇集后进行蛋白质提取和质谱分析。蛋白质鉴定基于四种数据库搜索（PEAKS、蛋白质组发现者软件、SearchGUI 软件和 PepExplorer）。使用 g:Profiler、Panther 和 String 平台进行了富集分析。在消除了搜索工具之间的识别冗余（基于标识符、肽段和跨数据库映射的三个层次）之后，在马胚胎样本中可靠地鉴定出了 1977 种蛋白质。蛋白质组分析揭示了 8 天马胚胎中旺盛的新陈代谢活动，参与 TCA 循环、ATP 生物合成和糖酵解等关键新陈代谢途径的大量蛋白质突显了这一点。高含量蛋白质中普遍含有伴侣蛋白，这表明蛋白质折叠和降解的调控是胚胎发育过程中的一个关键过程。这些发现为开发改进马胚胎培养基和优化体外受精技术的新策略铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.