Evaluation of circulating microRNA signature in patients with erosive hand osteoarthritis: The HOAmiR study

IF 7.2 2区医学 Q1 ORTHOPEDICS

Osteoarthritis and Cartilage Pub Date : 2024-07-08 DOI:10.1016/j.joca.2024.06.017

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引用次数: 0

Abstract

Objectives

To identify circulating micro-RNAs differentially expressed in patients with erosive hand osteoarthritis (HOA) compared to patients with non-erosive HOA and patients without HOA.

Methods

In the screening phase, 768 well-characterized micro-RNAs using Taqman low-density array cards were measured in 30 sera from 10 patients with erosive HOA, 10 patients with non-erosive HOA, and 10 controls without HOA, matched for age and body mass index (BMI). In a second step, we validated the micro-RNAs identified at the screening phase (adjusted p value < 0.05 after false discovery rate correction using Benjamini-Hochberg method and literature review) in larger samples (60 patients with erosive HOA and 60 patients without HOA matched for age and BMI).

Results

In the screening phase, we identified 21 down-regulated and 4 up-regulated micro-RNAs of interest between erosive HOA and control groups. Among these, 9 micro-RNAs (miR-373-3p, miR-558, miR-607, miR-653-5p, miR-137 and miR448 were down-regulated, and miR-142-3p, miR-144-3p and miR-34a-5p were up-regulated) were previously described in chondrocytes homeostasis or OA. We found only one significantly down-regulated micro-RNA between erosive and non-erosive HOA. In the validation phase, we showed replication of a single micro-RNA the significant downregulation of miR-196–5p, that had been previously identified in the screening phase among patients with erosive HOA compared to those without HOA.

After reviewing the literature and the miRNA-gene interaction prediction model, we found that this microRNA could interact with bone homeostasis and HOXC8, which could explain its role in osteoarthritis.

Conclusions

We found that miR-196-5p was down-regulated in patients with erosive HOA and some of its targets could explain a role in OA.

查看原文本刊更多论文

评估侵蚀性手部骨关节炎患者的循环 microRNA 特征：HOAmiR 研究。

目的与非侵蚀性手部骨关节炎（HOA）患者和无HOA患者相比，确定侵蚀性手部骨关节炎（HOA）患者循环中表达不同的微RNA：在筛选阶段，使用 Taqman 低密度阵列卡对 10 名侵蚀性手部骨关节炎患者、10 名非侵蚀性手部骨关节炎患者和 10 名未患手部骨关节炎的对照组的 30 份血清中的 768 种特征明确的微 RNA 进行了检测，这些患者的年龄和体重指数与对照组相匹配。第二步，我们在更大的样本（年龄和体重指数匹配的 60 名侵蚀性 HOA 患者和 60 名非 HOA 患者）中验证了筛查阶段发现的微 RNA（使用 Benjamini-Hochberg 方法和文献综述校正误发现率后，调整后的 p 值小于 0.05）：在筛选阶段，我们在侵蚀性 HOA 和对照组之间发现了 21 个下调和 4 个上调的相关微 RNA。其中，9 个微 RNA（miR-373-3p、miR-558、miR-607、miR-653-5p、miR-137 和 miR448 下调，miR-142-3p、miR-144-3p 和 miR-34a-5p 上调）以前在软骨细胞平衡或 OA 中被描述过。我们发现，在侵蚀性 HOA 和非侵蚀性 HOA 之间，只有一种微 RNA 明显下调。在验证阶段，我们重复了先前在筛查阶段发现的侵蚀性 HOA 患者与非侵蚀性 HOA 患者相比，miR-196-5p 明显下调的单一微 RNA。在查阅文献和miRNA-基因相互作用预测模型后，我们发现该microRNA可与骨稳态和HOXC8相互作用，这可以解释其在骨关节炎中的作用：我们发现，miR-196-5p 在侵蚀性 HOA 患者中被下调，而它的一些靶点可解释其在 OA 中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Osteoarthritis and Cartilage 医学-风湿病学

CiteScore

11.70

自引率

7.10%

发文量

802

审稿时长

52 days

期刊介绍： Osteoarthritis and Cartilage is the official journal of the Osteoarthritis Research Society International. It is an international, multidisciplinary journal that disseminates information for the many kinds of specialists and practitioners concerned with osteoarthritis.