EquCab_Finn: A new reference genome assembly for the domestic horse, Finnhorse

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-10 DOI:10.1111/age.13463

Kisun Pokharel, Melak Weldenegodguad, Tiina Reilas, Juha Kantanen

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Abstract

Finnhorse is Finland's native and national horse breed and it has genetic affinities to northern European and Asian horses. It has historical importance for agriculture, forest work and transport and as a war horse. Finnhorse has four breeding sections in the studbook and is under conservation and characterisation efforts. We sequenced and annotated the genome of a Finnhorse mare from the working horse section using PacBio and Omni-C data. This genome can complement the existing Thoroughbred reference genome (EquCab 3.0) and facilitate genetic studies of horses from northern Eurasia. We assembled 2.4 Gb of the genome with an N50 scaffold length of 83.8 Mb and the genome annotation resulted in a total of 19 748 protein coding genes of which 1200 were Finnhorse specific. The assembly has high quality and synteny with the current horse reference genome. We manually curated five genes of interest and deposited the final assembly in the European Nucleotide Archive under the accession no. PRJEB71364.

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EquCab_Finn：家马芬马的新参考基因组组装。

芬兰马是芬兰本土的国家马种，与北欧和亚洲的马有亲缘关系。它在农业、森林作业、运输以及作为战马方面具有重要的历史意义。芬兰马在种马目录中有四个繁殖区，目前正在进行保护和特征描述工作。我们使用 PacBio 和 Omni-C 数据对来自工作马区的一匹芬霍马母马的基因组进行了测序和注释。该基因组可以补充现有的纯血马参考基因组（EquCab 3.0），促进对欧亚大陆北部马匹的遗传研究。我们组装了 2.4 Gb 的基因组，N50 支架长度为 83.8 Mb，通过基因组注释共获得 19 748 个蛋白质编码基因，其中 1200 个为芬兰马特异基因。基因组注释共得到 19 748 个蛋白质编码基因，其中 1200 个是芬兰马特有的。该基因组与当前的马参考基因组具有很高的质量和同源性。我们对五个感兴趣的基因进行了人工标注，并将最终组装结果存入欧洲核苷酸档案，登录号为 PRJEB71364。PRJEB71364。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.