Two spurge species, Euphorbia resinifera O. Berg and Euphorbia officinarum subsp. echinus (Hook.f. & Coss.) Vindt inhibit colon cancer

2区医学 Q1 Medicine

BMC Complementary and Alternative Medicine Pub Date : 2024-07-10 DOI:10.1186/s12906-024-04566-3

Rania Benjamaa, Anlin Zhu, Soeun Kim, Dohyang Kim, Abdel Khalid Essamadi, Abdelkarim Moujanni, Anass Terrab, Namki Cho, Jaewoo Hong

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Abstract

Colon cancer, a prominent contributor to global cancer-related deaths, prompts the need for innovative treatment strategies. Euphorbia resinifera O. Berg (E. resinifera) and Euphorbia officinarum subsp. echinus Hook. f. & Coss Vindt (E. echinus) and their bee-derived products have been integral to traditional Moroccan medicine due to their potential health benefits. These plants have historical use in addressing various health issues, including cancer. However, their effects against colon cancer remain unclear, and the specific mechanisms underlying their anti-cancer effects lack comprehensive investigation. The study aimed to assess the potential anti-cancer effects of Euphorbia extract on colon cancer cell lines (DLD-1) through various techniques. The apoptosis, migration, and proliferation of DLD-1 cells were measured in DLD-1 cells. In addition, we conducted High-Performance Liquid Chromatography (HPLC) analysis to identify the profile of phenolic compounds present in the studied extracts. The extracts demonstrated inhibition of colon cancer cell migration. E. resinifera flower and E. echinus stem extracts show significant anti-migratory effects. Regarding anti-proliferative activity, E. resinifera flower extract hindered proliferation, whereas E. echinus flower extract exhibited dose-dependent inhibition. Apoptosis assays revealed E. resinifera flower extract inducing early-stage apoptosis and E. echinus flower extract promoting late-stage apoptosis. While apoptotic protein expression indicated, E. resinifera stem and propolis extracts had minimal impact on apoptosis. The findings provide evidence supporting the beneficial effects of E resinifera and E. echinus extracts on colon cancer and exerting anti-cancer properties.

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两种刺玫--Euphorbia resinifera O. Berg 和 Euphorbia officinarum subsp.

结肠癌是导致全球癌症相关死亡的主要原因之一，因此需要创新的治疗策略。Euphorbia resinifera O. Berg（E. resinifera）和 Euphorbia officinarum subsp.这些植物在解决包括癌症在内的各种健康问题方面有着悠久的历史。然而，它们对结肠癌的作用仍不明确，其抗癌作用的具体机制也缺乏全面的研究。本研究旨在通过各种技术评估大戟提取物对结肠癌细胞株（DLD-1）的潜在抗癌作用。我们测定了 DLD-1 细胞的凋亡、迁移和增殖。此外，我们还进行了高效液相色谱（HPLC）分析，以确定所研究提取物中存在的酚类化合物的概况。这些提取物都具有抑制结肠癌细胞迁移的作用。E.resinifera花和E.echinus茎提取物具有显著的抗迁移作用。在抗增殖活性方面，E. resinifera 花提取物阻碍了细胞的增殖，而 E. echinus 花提取物则表现出剂量依赖性抑制作用。细胞凋亡试验表明，树脂花提取物可诱导早期细胞凋亡，而刺五加花提取物则可促进晚期细胞凋亡。凋亡蛋白的表达表明，树脂草茎提取物和蜂胶提取物对细胞凋亡的影响很小。这些研究结果为树脂草和刺五加提取物对结肠癌的有益影响和抗癌特性提供了证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Complementary and Alternative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

7.00

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： BMC Complementary Medicine and Therapies is an open access journal publishing original peer-reviewed research articles on interventions and resources that complement or replace conventional therapies, with a specific emphasis on research that explores the biological mechanisms of action, as well as their efficacy, safety, costs, patterns of use and/or implementation.