Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9

IF 9.3 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Integrative Plant Biology Pub Date : 2024-07-10 DOI:10.1111/jipb.13741

Chenglu Zhao, Huanhuan Lou, Qian Liu, Siqi Pei, Qiansheng Liao, Zhenghe Li

引用次数: 0

Abstract

Tomato spotted wilt virus-mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA-free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.

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利用 RNA 病毒介导的 CRISPR/Cas9 在辣椒中进行高效、无转化的基因组编辑。

番茄斑萎病毒介导的CRISPR/Cas9传递绕过了稳定转化的需要，允许在辣椒中进行高效、无DNA的基因组编辑。值得注意的是，高达 77.9% 的再生辣椒植株含有遗传编辑。该方法已通过两个辣椒品种的验证，并与现有的组织培养方案兼容。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Integrative Plant Biology 生物-生化与分子生物学

CiteScore

18.00

自引率

5.30%

发文量

220

审稿时长

3 months

期刊介绍： Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.