Izabella de Carvalho Batista Muniz , Jonathan Barbosa Santos , Rosana Moura de Oliveira , Fernanda Godoy Santos , Evaldo Cardozo de Souza Junior , Linda Oyama , Rafael da Costa Ilhéu Fontan , Renata Cristina Ferreira Bonomo
{"title":"Current advances in obtaining novel cyclodextrin glycosyltransferases for optimizing the synthesis of cyclodextrins","authors":"Izabella de Carvalho Batista Muniz , Jonathan Barbosa Santos , Rosana Moura de Oliveira , Fernanda Godoy Santos , Evaldo Cardozo de Souza Junior , Linda Oyama , Rafael da Costa Ilhéu Fontan , Renata Cristina Ferreira Bonomo","doi":"10.1016/j.procbio.2024.07.008","DOIUrl":null,"url":null,"abstract":"<div><p>The cyclodextrins (CDs) market has increased due to their numerous applications, especially in the food and pharmaceutical industries. The high cost of synthetizing one unique form of CDs, mainly the α- and γ- forms, hinders their use in certain processes. Since CDs are exclusively produced by the enzymatic conversion of starch or starch derivates using cyclodextrin glycosyltransferase (CGTase), enhancing this enzyme production means feasible CD synthesis. Recent discoveries of novel CGTase producers and strategies for optimizing fermentation processes and immobilization techniques have expanded its applications. However, the low yield arising from wild strains remains an obstacle. Studies focused on overexpressing CGTase through heterologous expression and improving their catalytic properties by altering protein structure using protein engineering approaches have been explored to enable upscaling the applications of CGTase. This review surveys recent approaches employed in CGTases production aiming at industrial scale. Overall, the importance of advances in biotechnology for cost reduction in upscale processes and perspectives for future research were highlighted.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324002290","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The cyclodextrins (CDs) market has increased due to their numerous applications, especially in the food and pharmaceutical industries. The high cost of synthetizing one unique form of CDs, mainly the α- and γ- forms, hinders their use in certain processes. Since CDs are exclusively produced by the enzymatic conversion of starch or starch derivates using cyclodextrin glycosyltransferase (CGTase), enhancing this enzyme production means feasible CD synthesis. Recent discoveries of novel CGTase producers and strategies for optimizing fermentation processes and immobilization techniques have expanded its applications. However, the low yield arising from wild strains remains an obstacle. Studies focused on overexpressing CGTase through heterologous expression and improving their catalytic properties by altering protein structure using protein engineering approaches have been explored to enable upscaling the applications of CGTase. This review surveys recent approaches employed in CGTases production aiming at industrial scale. Overall, the importance of advances in biotechnology for cost reduction in upscale processes and perspectives for future research were highlighted.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.