Upstream Process Protocol for MSCs Isolated from Different Human-Based Tissue Origins.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-07-06 DOI:10.1007/7651_2024_553

Pelin Kılıç, Cansu Özdemir, Begüm Coşar, Büşra Nigar Savran, Aysun Sarıkaya, Begüm Sargon, Alım Toprakkale, İrem Songür, Özlem Kandemir Seçgin, Pınar Akpınar Oktar, Elif NazIı Çetindağ, Deniz Yurtsever Sarıca, Serpil Taşdelen, Üstün Ezer, Ahmet Emin Kürekçi, Günhan Gürman

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引用次数: 0

Abstract

This chapter introduces the increasing significance of mesenchymal stromal/stem cell (MSC) production in regenerative medicine and cellular therapeutics, outlines the growing interest in MSCs for various medical applications, and highlights their potential in advanced therapy medicinal products (ATMPs) and the advancements in cell culture technologies that have facilitated large-scale MSC production under Good Manufacturing Practices (GMP), ensuring safety and efficacy. This chapter describes an optimized upstream protocol for laboratory-scale MSC production from different tissue sources. This protocol, conducted in flasks, controls critical parameters and lays the foundation for downstream processing to generate ATMPs. This comprehensive approach underscores the potential of MSCs in clinical applications and the importance of tailored production processes.

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从不同人体组织来源分离的间充质干细胞的上游工艺规程。

本章介绍了间充质基质/干细胞（MSC）生产在再生医学和细胞疗法中日益重要的意义，概述了间充质干细胞在各种医疗应用中日益增长的兴趣，并重点介绍了间充质干细胞在高级治疗药物产品（ATMP）中的潜力，以及细胞培养技术的进步，这些进步促进了间充质干细胞在良好生产规范（GMP）下的大规模生产，确保了安全性和有效性。本章介绍了从不同组织来源进行实验室规模间充质干细胞生产的优化上游方案。该方案在烧瓶中进行，控制关键参数，为下游处理生成 ATMP 奠定基础。这种综合方法强调了间充质干细胞在临床应用中的潜力以及定制生产工艺的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.