Phenotypic and Molecular Profiling of Vitis Vinifera L. Genotypes Against Anthracnose Through SCAR Markers

Abstract

Grape is one of the most important fruit crop in the world. It is used for eating raw as well as for wine making in different countries of the world. In countries where consumer prefer seedless grape, there is more trend for growing table grapes. Seedless grapes are highly susceptible to fungal disease, so experiment was designed to screen different grape genotypes for anthracnose. Screening of different grape genotypes was done by artificial inoculation method as well as by using molecular markers. For phenotypic screening, inoculum was prepared form already infected leaves, and spore concentrations of 10⁵ spores/ml was applied on healthy leaves of different grape genotypes. Four days after inoculation disease symptoms were observed and disease intensity was measured by using disease scoring scale. Genotype Daakh is highly resistant to Anthracnose. Genotypes NARC Black, White vine, Himroid and King’s Ruby are considered as moderately resistant while genotypes Sugra One, Like Mount, and Vitro Black considered showed Susceptibility to anthracnose. Molecular screening was done by using SCAR makers linked to anthracnose. Screened disease resistant genotypes can be used for introgression of resistant gene into susceptible cultivars for development of cultivars with stronger resistance against anthracnose.