Guideline for the verification of digital PCR methods in analytical GMO testing

IF 1.4 3区农林科学 Q4 FOOD SCIENCE & TECHNOLOGY

Journal of Consumer Protection and Food Safety Pub Date : 2024-06-25 DOI:10.1007/s00003-024-01516-6

Christopher Weidner, Jakob Frenzel, Daniela Bartsch, Hans-Ulrich Waiblinger, Joachim Mankertz

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引用次数: 0

Abstract

The international working group “Development of methods for identification of foodstuffs produced by means of genetic engineering techniques” was established pursuant to § 64 of the German Food and Feed Code (LFGB). It has developed a guideline for implementing and verifying digital PCR methods previously validated as real-time PCR techniques. While event-specific methods in testing of genetically modified organisms (GMO) have traditionally relied on real-time PCR, new digital PCR technologies offer significant advantages and therefore, leading to an increased use by control laboratories. This guideline provides practical recommendations for transferring the real-time PCR to digital PCR and for verifying the digital PCR method. The guideline is applicable to analysis of GMO in food, feed and seed and is freely available on the website of the Federal Office of Consumer Protection and Food Safety (BVL).

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转基因生物分析检测中数字 PCR 方法验证指南

根据《德国食品和饲料法典》（LFGB）第 64 条，成立了 "基因工程技术食品鉴别方法开发 "国际工作组。该工作组为实施和验证先前作为实时 PCR 技术验证的数字 PCR 方法制定了指导原则。虽然转基因生物 (GMO) 检测中的特定事件检测方法传统上依赖于实时 PCR，但新的数字 PCR 技术具有显著优势，因此控制实验室越来越多地使用这种方法。本指南提供了将实时 PCR 转为数字 PCR 以及验证数字 PCR 方法的实用建议。该指南适用于食品、饲料和种子中转基因生物的分析，可在联邦消费者保护和食品安全办公室 (BVL) 的网站上免费获取。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Consumer Protection and Food Safety Veterinary-Food Animals

CiteScore

3.70

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The JCF publishes peer-reviewed original Research Articles and Opinions that are of direct importance to Food and Feed Safety. This includes Food Packaging, Consumer Products as well as Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering. All peer-reviewed articles that are published should be devoted to improve Consumer Health Protection. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of Food and Feed Safety issues on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of Food and Feed Safety and Consumer Health Protection. Manuscripts – preferably written in English but also in German – are published as Research Articles, Reviews, Methods and Short Communications and should cover aspects including, but not limited to: · Factors influencing Food and Feed Safety · Factors influencing Consumer Health Protection · Factors influencing Consumer Behavior · Exposure science related to Risk Assessment and Risk Management · Regulatory aspects related to Food and Feed Safety, Food Packaging, Consumer Products, Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering · Analytical methods and method validation related to food control and food processing. The JCF also presents important News, as well as Announcements and Reports about administrative surveillance.