Pheretimoid earthworms of India: new insight from molecular systematics and phylogenetic relationship with new records

IF 1.9 3区 生物学 Q3 EVOLUTIONARY BIOLOGY
Nalini Tiwari, Samuel W. James, Shweta Yadav
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引用次数: 0

Abstract

Around the world, the spread of pheretimoid earthworm species from Southeast Asia has raised ecological, environmental, and conservation concerns in urban-suburban settings and forest habitats. There is currently a global discourse surrounding the taxonomic status of pheretimoid earthworms. Therefore, a comparative molecular systematics study was conducted on pheretimoid, utilizing an integrative taxonomy approach that relied on morpho-anatomical characterization and the COI gene. The study generated and compared the 51 COI barcodes of pheretimoid with existing 156 COI sequences of pheretimoid earthworms worldwide, with the primary goal of testing various analytical methods for species delimitation and examining phylogenetic reconstruction. Seven different methods of species delineation were applied, viz., Automatic Barcode Gap Discovery (ABGD), Assemble Species by Automatic Partitioning (ASAP), General Mixed Yule Coalescence (GMYC), Poisson Tree Processes (PTP), Bayesian Poisson Tree Processes (bPTP), Multiple Poisson Tree Processes (mPTP), and Refined Single Linkage (RESL). Additionally, trees using Bayesian Inference (BI) and Maximum Likelihood (ML) were created for phylogenetic studies. The results unveiled 10 species belonging to 3 genera, viz., Amynthas, Metaphire, and Polypheretima. Furthermore, the study reported one new record from India, Amynthas andersoni choprai, and range expansion of Amynthas alexandri, Metaphire posthuma, and Polypheretima elongata to Manipur, the north-eastern region of India. Further, the peregrine species Amynthas corticis was reported for the first time in Madhya Pradesh in the central part of India. The study concluded that the genera Amynthas, Metaphire, and Pheretima do not exhibit monophyletic lineages which corroborates with previous studies. The study aligns with earlier studies that recommended reassessing the numerical revision of the genus Pheretima auct. (Sims & Easton, 1972). In addition, a comprehensive analysis of the evolutionary relationships between multiple pheretimoid earthworms in India has been provided, laying the foundation for understanding their origins.

Abstract Image

印度的Pheretimoid蚯蚓:分子系统学的新见解以及与新记录的系统发育关系
在全球范围内,来自东南亚的哲毛蚯蚓物种的传播引起了人们对城市-郊区环境和森林栖息地的生态、环境和保护问题的关注。目前,全球范围内都在讨论拟蛭蚯蚓的分类地位。因此,我们利用综合分类方法,通过形态解剖特征和 COI 基因,对 pheretimoid 进行了分子系统学比较研究。该研究生成了 51 个 pheretimoid 的 COI 条形码,并将其与全球现有的 156 个 pheretimoid 蚯蚓的 COI 序列进行了比较,主要目的是测试物种划分的各种分析方法,并研究系统发育重建。应用了七种不同的物种划分方法,即自动条形码间隙发现(ABGD)、通过自动分区组合物种(ASAP)、通用混合尤利凝聚(GMYC)、泊松树过程(PTP)、贝叶斯泊松树过程(bPTP)、多重泊松树过程(mPTP)和精制单链(RESL)。此外,还利用贝叶斯推理(BI)和最大似然法(ML)创建了系统发生研究树。研究结果揭示了隶属于 3 个属的 10 个物种,即 Amynthas 属、Metaphire 属和 Polypheretima 属。此外,该研究还报告了印度的一个新记录--Amynthas andersoni choprai,以及Amynthas alexandri、Metaphire posthuma和Polypheretima elongata在印度东北部曼尼普尔的分布范围。此外,还首次在印度中部的中央邦报告了游隼物种Amynthas corticis。研究得出结论,Amynthas 属、Metaphire 属和 Pheretima 属并不表现为单系,这与之前的研究相吻合。该研究与之前的研究一致,之前的研究建议重新评估 Pheretima 属的数字修订(Sims & Easton)。(Sims & Easton, 1972)。此外,该研究还全面分析了印度多种蚯蚓之间的进化关系,为了解它们的起源奠定了基础。
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来源期刊
Organisms Diversity & Evolution
Organisms Diversity & Evolution 生物-进化生物学
CiteScore
3.60
自引率
0.00%
发文量
56
审稿时长
>12 weeks
期刊介绍: Organisms Diversity & Evolution (published by the Gesellschaft fuer Biologische Systematik, GfBS) is devoted to furthering our understanding of all aspects of organismal diversity and evolution. Papers addressing evolutionary aspects of the systematics, phylogenetics, morphology and development, taxonomy and biogeography of any group of eukaryotes, recent or fossil, are welcome. Priority is given to papers with a strong evolutionary and/or phylogenetic focus. Manuscripts presenting important methods or tools or addressing key theoretical, methodological, and philosophical principles related to the study of organismal diversity are also welcome. Species descriptions are welcome as parts of a manuscript of broader interest that strive to integrate such taxonomic information with the other areas of interest mentioned above.
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