Kush Kumar Yadav, Patricia A Boley, Carolyn M Lee, Saroj Khatiwada, Kwonil Jung, Thamonpan Laocharoensuk, Jake Hofstetter, Ronna Wood, Juliette Hanson, Scott P Kenney
{"title":"Rat hepatitis E virus (HEV) cross-species infection and transmission in pigs","authors":"Kush Kumar Yadav, Patricia A Boley, Carolyn M Lee, Saroj Khatiwada, Kwonil Jung, Thamonpan Laocharoensuk, Jake Hofstetter, Ronna Wood, Juliette Hanson, Scott P Kenney","doi":"10.1093/pnasnexus/pgae259","DOIUrl":null,"url":null,"abstract":"Strains of Rocahepevirus ratti, an emerging hepatitis E virus (HEV), have recently been found to be infectious to humans. Rats are a primary reservoir of the virus; thus, it is referred to as “rat HEV”. Rats are often found on swine farms in close contact with pigs. Our goal was to determine whether swine may serve as a transmission host for zoonotic rat HEV by characterizing an infectious cDNA clone of a zoonotic rat HEV, strain LCK-3110, in vitro and in vivo. RNA transcripts of LCK-3110 were constructed and assessed for their replicative capacity in cell culture and in gnotobiotic pigs. Fecal suspension from rat HEV-positive gnotobiotic pigs was inoculated into conventional pigs cohoused with naïve pigs. Our results demonstrated that capped RNA transcripts of LCK-3110 rat HEV replicated in vitro and successfully infected conventional pigs that transmit the virus to cohoused animals. The infectious clone of rat HEV may afford an opportunity to study the genetic mechanisms of rat HEV cross-species infection and tissue tropism.","PeriodicalId":516525,"journal":{"name":"PNAS Nexus","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PNAS Nexus","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/pnasnexus/pgae259","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Strains of Rocahepevirus ratti, an emerging hepatitis E virus (HEV), have recently been found to be infectious to humans. Rats are a primary reservoir of the virus; thus, it is referred to as “rat HEV”. Rats are often found on swine farms in close contact with pigs. Our goal was to determine whether swine may serve as a transmission host for zoonotic rat HEV by characterizing an infectious cDNA clone of a zoonotic rat HEV, strain LCK-3110, in vitro and in vivo. RNA transcripts of LCK-3110 were constructed and assessed for their replicative capacity in cell culture and in gnotobiotic pigs. Fecal suspension from rat HEV-positive gnotobiotic pigs was inoculated into conventional pigs cohoused with naïve pigs. Our results demonstrated that capped RNA transcripts of LCK-3110 rat HEV replicated in vitro and successfully infected conventional pigs that transmit the virus to cohoused animals. The infectious clone of rat HEV may afford an opportunity to study the genetic mechanisms of rat HEV cross-species infection and tissue tropism.
最近发现，一种新出现的戊型肝炎病毒（HEV）--Rocahepevirus ratti 株系可传染给人类。大鼠是该病毒的主要贮存者，因此被称为 "大鼠戊型肝炎病毒"。猪场中经常发现老鼠与猪密切接触。我们的目标是通过研究人畜共患病大鼠 HEV（LCK-3110 株）感染性 cDNA 克隆的体外和体内特征，确定猪是否可能成为人畜共患病大鼠 HEV 的传播宿主。构建了 LCK-3110 的 RNA 转录本，并评估了其在细胞培养和无饲养动物猪体内的复制能力。将大鼠 HEV 阳性无生物猪的粪悬液接种到与天真猪同群的常规猪体内。我们的研究结果表明，LCK-3110 大鼠 HEV 的封顶 RNA 转录本可在体外复制，并成功感染常规猪，将病毒传播给同群动物。大鼠 HEV 的感染性克隆可能为研究大鼠 HEV 跨物种感染和组织滋养的遗传机制提供了机会。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
