Biopharmaceutical beamline TLS 15A1 for macromolecular crystallography at the National Synchrotron Radiation Research Center

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-21 DOI:10.1002/jccs.202400111

Cheng-Hung Chiang, Chung-Kuang Chou, Chien-Chang Tseng, Yi-Hui Chen, Yi-Chun Liu, Chen-Ying Huang, Chun-Hsiung Chao, Chun-Hsiang Huang

引用次数: 0

Abstract

Synchrotron protein crystallography (PX) remains an indispensable tool for uncovering the atomic structures of biomolecules. TLS 15A1, operated by the protein diffraction group of NSRRC, has been accessible to both academic and industrial users since January 2013. This beamline is energy-tunable within a range between 5.6 and 15.5 keV, which is suitable for phasing experiments. Over the past 11 years, TLS 15A1 has supported 657 projects and facilitated 1523 experiments across 41,656 h of beamtime. This article describes the beamline layout, studies of factors influencing data quality, and significant scientific contributions.

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国家同步辐射研究中心用于大分子晶体学的生物制药光束线 TLS 15A1

同步加速器蛋白质晶体学（PX）仍然是揭示生物大分子原子结构不可或缺的工具。由国家科学研究中心蛋白质衍射组运行的TLS 15A1光束线自2013年1月起向学术界和工业界用户开放。该光束线可在 5.6 至 15.5 keV 范围内进行能量调谐，适用于相位实验。在过去的11年中，TLS 15A1支持了657个项目，促进了1523项实验，光束时间达41656小时。本文介绍了光束线布局、影响数据质量因素的研究以及重大科学贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.