Sevoflurane inhibits the malignant behavior of nasopharyngeal carcinoma cells by regulating mitochondrial membrane potential.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-01 DOI:10.4149/gpb_2024014

Xuan Peng, YuLin Zhou

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Abstract

This study aims to determine the effect of sevoflurane (Sev) on nasopharyngeal carcinoma (NPC) in malignant behavior and mitochondrial membrane potential (MMP). NPC cells (5-8F and CNE2) were exposed to Sev at different concentrations and then tested for proliferation by CCK-8 and colony formation assays, apoptosis by flow cytometry, and invasion and migration by Transwell assays. In addition, the Warburg effect was examined by measurements of glucose consumption, lactic acid production, and adenosine triphosphate (ATP). Mitochondrial function was evaluated by reactive oxygen species (ROS) production, oxidative stress-related indexes, and mitochondrial membrane potential. Sev suppressed 5-8F and CNE2 cell proliferation, invasion, and migration, and enhanced apoptosis. Moreover, Sev dampened the Warburg effect by reducing glucose consumption, lactic acid production, and ATP, as well as decreasing hexokinase 2 and pyruvate kinases type M2 protein expressions. Also, Sev induced ROS production and malondialdehyde content and reduced superoxide and glutathione peroxidase levels. Finally, Sev caused damage to mitochondrial homeostasis through induction of cleaved caspase-3, cleaved caspase-9, and cytochrome c protein expression and reduction of MMP. Sev inhibits the malignant behavior of NPC cells by regulating MMP.

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七氟烷通过调节线粒体膜电位抑制鼻咽癌细胞的恶性行为

本研究旨在确定七氟烷（Sev）对鼻咽癌（NPC）恶性行为和线粒体膜电位（MMP）的影响。将鼻咽癌细胞（5-8F 和 CNE2）暴露于不同浓度的 Sev，然后用 CCK-8 和集落形成试验检测细胞增殖情况，用流式细胞仪检测细胞凋亡情况，用 Transwell 试验检测细胞侵袭和迁移情况。此外，还通过测量葡萄糖消耗、乳酸生成和三磷酸腺苷（ATP）来检测沃伯格效应。线粒体功能通过活性氧（ROS）产生、氧化应激相关指数和线粒体膜电位进行评估。Sev抑制了5-8F和CNE2细胞的增殖、侵袭和迁移，并增强了细胞凋亡。此外，Sev 通过减少葡萄糖消耗、乳酸生成和 ATP，以及降低己糖激酶 2 和丙酮酸激酶 M2 型蛋白表达，抑制了沃伯格效应。此外，Sev 还会诱导 ROS 生成和丙二醛含量，并降低超氧化物和谷胱甘肽过氧化物酶的水平。最后，Sev 通过诱导裂解的 caspase-3、裂解的 caspase-9 和细胞色素 c 蛋白的表达以及降低 MMP，对线粒体平衡造成破坏。Sev通过调节MMP抑制鼻咽癌细胞的恶性行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.