Evidence of comammox bacteria playing a dominant role in Lake Taihu sediments based on metagenomic analysis

Abstract

The nitrification process plays an important role in the nitrogen cycle, in which the ammonia-oxidation process mediated by microorganisms is the rate-limiting step. Environmental factors can affect the distribution and activity of ammonia-oxidizing microorganisms (AOM), including ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), and complete ammonia oxidizer (comammox Nitrospira). At present, most studies have used amoA as a marker gene for the ammonia oxidation process to analyze the differences among AOM community composition and abundance in the environment. In this study, metagenomic sequencing was used to study the differences in community composition and functional gene distribution of nitrifying microorganisms in the sediments of Lake Taihu with different eutrophication levels. It was found that comammox Nitrospira and typical nitrite oxidizer NOB Nitrospira, which belong to Nitrospirota, had higher relative abundance at most sites compared to AOB and AOA. Furthermore, the network analysis of genes related to nitrogen cycle showed that the main survival mode of nitrogen metabolizing microorganisms was mutualism. Besides, the microbial genomes in the sediments of Lake Taihu were reconstructed by metagenomic binning, which showed that among the 167 bins obtained, 2 bins (bin 9 and bin 32) were annotated as comammox Nitrospira, and had high abundance in the macrophytes-dominated lakes (such as South Lake Taihu and West Coast). In addition, bin 9, which belongs to comammox Nitrospira, annotates amoA genes associated with ammonia oxidation, and other genes associated with urea decomposition and transport, suggesting functional diversity. Overall, these findings suggest that AOM have different distribution characteristics, among which comammox Nitrospira has high diversity and may be potentially dominant in macrophytes-dominated lakes.