Resolution in super-resolution microscopy — definition, trade-offs and perspectives

IF 81.3 1区生物学 Q1 CELL BIOLOGY

Nature Reviews Molecular Cell Biology Pub Date : 2024-07-01 DOI:10.1038/s41580-024-00755-7

Kirti Prakash, David Baddeley, Christian Eggeling, Reto Fiolka, Rainer Heintzmann, Suliana Manley, Aleksandra Radenovic, Carlas Smith, Hari Shroff, Lothar Schermelleh

引用次数: 0

Abstract

Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM. In this Viewpoint, experts discuss resolution and common trade-offs in super-resolution microscopy, aiming to improve how biologists use the technology.

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超分辨率显微镜的分辨率--定义、权衡与展望。

超分辨显微镜（SRM）在生物科学领域越来越受欢迎；然而，有关光学分辨率的说法却存在争议，而且往往具有误导性。在本视点中，专家们分享了他们对分辨率和常见权衡（如标记和后处理）的看法，旨在为生物学家澄清这些问题，促进对超分辨率显微镜的深入理解和最佳利用。在本视点中，专家们讨论了超分辨率显微镜的分辨率和常见权衡，旨在改进生物学家使用该技术的方式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Reviews Molecular Cell Biology 生物-细胞生物学

CiteScore

173.60

自引率

0.50%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Nature Reviews Molecular Cell Biology is a prestigious journal that aims to be the primary source of reviews and commentaries for the scientific communities it serves. The journal strives to publish articles that are authoritative, accessible, and enriched with easily understandable figures, tables, and other display items. The goal is to provide an unparalleled service to authors, referees, and readers, and the journal works diligently to maximize the usefulness and impact of each article. Nature Reviews Molecular Cell Biology publishes a variety of article types, including Reviews, Perspectives, Comments, and Research Highlights, all of which are relevant to molecular and cell biologists. The journal's broad scope ensures that the articles it publishes reach the widest possible audience.