3-chloro-1,2-propanediol induces oxidative stress and promotes testicular damage and infertility in rats through CYP2C9

IF 3.3 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2024-06-27 DOI:10.1016/j.reprotox.2024.108633

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引用次数: 0

Abstract

3-chloro-1,2-propanediol (3-MCPD) is a newly discovered food process pollutant with nephrotoxicity. And the mechanism by which 3-MCPD affects male spermatogenesis has not been fully studied. Cell viability, blood-testis barrier (BTB) related protein, progesterone content, reactive oxygen species (ROS) generation, and cell apoptosis were determined by a CCK8 assay, western blot, ELISA, flow cytometry, and TUNEL staining, respectively. Wistar rats were divided into three groups: low-dose 3-MCPD, high-dose 3-MCPD, and control. Sperm parameters, hormonal levels, and biomarkers of oxidative stress in the testis and epididymis were detected by ELISA. Multiple molecular experiments including molecular docking and western blot were used to elucidate the underlying mechanisms. 3-MCPD affects testicular cell activity, and promotes ROS production and apoptosis. Disrupting the integrity of BTB in the body, downregulating sex hormones and sperm quality, and promoting apoptosis. 3-MCPD may function through CYP2C9. This study preliminarily explores the mechanism by which 3-MCPD affects spermatogenesis. It was found that 3-MCPD destroys the structure and function of BTB and damages the testicular function of male mice, thus affecting the process of spermatogenesis via CYP2C9.

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3-氯-1,2-丙二醇通过 CYP2C9 诱导氧化应激，促进大鼠睾丸损伤和不育。

3-氯-1,2-丙二醇（3-MCPD）是一种新发现的具有肾毒性的食品加工污染物。3-MCPD 影响男性精子生成的机制尚未得到充分研究。研究采用 CCK8 检测法、Western 印迹法、ELISA 法、流式细胞术和 TUNEL 染色法分别测定了细胞活力、血睾屏障（BTB）相关蛋白、孕酮含量、活性氧（ROS）生成和细胞凋亡。Wistar 大鼠被分为三组：低剂量 3-氯丙二醇组、高剂量 3-氯丙二醇组和对照组。通过 ELISA 检测精子参数、激素水平以及睾丸和附睾中氧化应激的生物标志物。研究还采用了多种分子实验（包括分子对接和 Western 印迹）来阐明其潜在机制。氯丙二醇会影响睾丸细胞的活性，促进 ROS 生成和细胞凋亡。破坏体内 BTB 的完整性，下调性激素和精子质量，并促进细胞凋亡。3-MCPD 可能通过 CYP2C9 发挥作用。这项研究初步探讨了 3-MCPD 影响精子生成的机制。研究发现，氯丙二醇会破坏 BTB 的结构和功能，损害雄性小鼠的睾丸功能，从而通过 CYP2C9 影响精子生成过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.

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