Effect of hydro-alcoholic extract of Nigella sativa on cisplatin-induced memory impairment and brain oxidative stress status in male rats.

IF 1.9 Q3 CHEMISTRY, MEDICINAL
Yasin Mahmoud Janloo, Fatemeh Sadat Attari, Sahar Roshan, Hadi Lotfi, Amir Hossein Pezeshki, Masoud Hosseinzadeh, Batool Shakiba-Jam, Marzieh Kafami
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引用次数: 0

Abstract

Objective: Studies have shown the complications of chemotherapy on learning and memory. Empirical evidence suggests that Nigella sativa (NS) has neuroprotective activities. Therefore, the aim of our study was to investigate the effects of NS on cisplatin-induced memory impairment.

Materials and methods: This study was conducted on 40 male rats grouped as: control (saline: 2 ml/kg, intraperitoneally (IP), once weekly/2 weeks), cisplatin (Cis, 2 mg/kg, IP, once weekly/2 weeks), NS (200 mg/kg, IP, once weekly/2 weeks), Cis +NS 200 (2 mg/kg Cis + 200 mg/kg NS, IP, once weekly/2 weeks), and Cis +NS 400 (2 mg/kg Cis + 400 mg/kg NS, IP, once weekly/2 weeks). Morris water maze (MWM) test was used to assess spatial learning and memory. In addition, superoxide dismutase (SOD) activity, and thiol and malondialdehyde (MDA) levels were evaluated in the brain.

Results: Cis significantly enhanced the traveled distance and time spent in the target quadrant in the MWM test. Additionally, MDA levels increased in the Cis group, while thiol and SOD decreased in this group. As a result of treatment with NS, behavioral results were reversed in the groups receiving NS compared to the Cis group. Also, NS reduced MDA level but improved SOD and thiol levels in brain tissue samples.

Conclusion: NS could improve memory impairment and oxidative stress in animals receiving Cis. Therefore, NS could be used as a potential food supplement to prevent neurotoxicity in patients undergoing chemotherapy.

黑麦草水醇提取物对顺铂诱导的雄性大鼠记忆损伤和脑氧化应激状态的影响
研究目的研究表明,化疗会对学习和记忆产生并发症。经验证据表明,黑麦草(Nigella sativa,NS)具有神经保护活性。因此,我们的研究旨在探讨 Nigella sativa 对顺铂诱导的记忆损伤的影响:本研究对 40 只雄性大鼠进行了分组:对照组(生理盐水:2 毫升/千克,腹腔注射(IP),每周一次/两周)、顺铂(Cis,2 毫克/千克,IP,每周一次/两周)、NS(200 毫克/千克,IP,每周一次/两周)、Cis +NS 200(2 毫克/千克 Cis + 200 毫克/千克 NS,IP,每周一次/两周)和 Cis +NS 400(2 毫克/千克 Cis + 400 毫克/千克 NS,IP,每周一次/两周)。莫里斯水迷宫(MWM)测试用于评估空间学习和记忆。此外,还对大脑中的超氧化物歧化酶(SOD)活性、硫醇和丙二醛(MDA)水平进行了评估:结果:在 MWM 测试中,Cis 能明显提高在目标象限的移动距离和时间。此外,顺式组的 MDA 水平升高,而硫醇和 SOD 水平下降。与顺式组相比,NS治疗组的行为结果发生了逆转。此外,NS还降低了脑组织样本中的MDA水平,但提高了SOD和硫醇水平:结论:NS 可改善接受顺式疗法的动物的记忆损伤和氧化应激。因此,NS 可作为一种潜在的食物补充剂,用于预防化疗患者的神经毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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