A tricalcium phosphate (TCP) and hydroxyapatite (HA) based light-cured biomaterial for vital pulp therapy

IF 4.6 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
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Abstract

Objectives

Evaluate a new light-cured material with better properties for vital pulp therapy.

Methods

Light-cured resin materials consisted of polyethylene glycol (600) diacrylate mixed with different ratios of TCP to HA. In addition to the temperature change (n = 5 for each subgroup) were tested, cell viability and Alizarin Red Staining (ARS) assay were also tested in vitro on human dental pulp cells (n = 6 for each subgroup). Lastly, the material was then compared with Biodentine and control groups in the molars of Wistar rats in vivo for histology assessment.

Results

The temperature change for the new materials were under 5 degrees Celsius. For the in vitro assessments, there was no significant difference on day 3 and day 7 for cell viability test. ARS assay showed significantly higher mineralized nodule formation when treated without induction medium for Group D and Biodentine on day 10 compared to Group C and control. On the contrary, Biodentine and control groups treated with induction medium showed significant higher mineralization than the new materials. Histology assessments demonstrated higher mineralized content in Group D and Biodentine on week 3 and week 6. The inflammatory cells in the dental pulp complex of the Biodentine group resolved on week 6 while the inflammation resolved in Group D on week 3.

Significance

The new material exhibits low heat production, low cytotoxicity, and good calcium ion release capability. Compared to traditional materials, it has shorter setting time and better aesthetic outcomes, making it highly suitable for use in vital pulp therapy.

一种基于磷酸三钙(TCP)和羟基磷灰石(HA)的光固化生物材料,用于牙髓治疗。
目的评估一种性能更好的新型光固化材料,用于牙髓治疗:光固化树脂材料由聚乙二醇(600)二丙烯酸酯与不同比例的 TCP 和 HA 混合组成。除了测试温度变化(每个子组 5 个)外,还对人牙髓细胞(每个子组 6 个)进行了细胞活力和茜素红染色(ARS)体外测试。最后,在 Wistar 大鼠的臼齿中将材料与 Biodentine 和对照组进行体内比较,以进行组织学评估:结果:新材料的温度变化低于 5 摄氏度。在体外评估中,第 3 天和第 7 天的细胞存活率测试没有明显差异。ARS 检测显示,与 C 组和对照组相比,D 组和 Biodentine 在第 10 天不使用诱导培养基处理时,矿化结核形成率明显更高。相反,使用诱导培养基处理的 Biodentine 组和对照组的矿化度明显高于新材料组。组织学评估显示,D 组和 Biodentine 在第 3 周和第 6 周的矿化含量更高。Biodentine 组牙髓复合体中的炎症细胞在第 6 周消退,而 D 组的炎症在第 3 周消退:意义:新材料产热低、细胞毒性低、钙离子释放能力强。与传统材料相比,它的固化时间更短,美观效果更好,非常适合用于牙髓治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Dental Materials
Dental Materials 工程技术-材料科学:生物材料
CiteScore
9.80
自引率
10.00%
发文量
290
审稿时长
67 days
期刊介绍: Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.
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