BTA2 regulates tiller angle and the shoot gravity response through controlling auxin content and distribution in rice

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhen Li, Junhua Ye, Qiaoling Yuan, Mengchen Zhang, Xingyu Wang, Jing Wang, Tianyi Wang, Hongge Qian, Xinghua Wei, Yaolong Yang, Lianguang Shang, Yue Feng
{"title":"BTA2 regulates tiller angle and the shoot gravity response through controlling auxin content and distribution in rice","authors":"Zhen Li,&nbsp;Junhua Ye,&nbsp;Qiaoling Yuan,&nbsp;Mengchen Zhang,&nbsp;Xingyu Wang,&nbsp;Jing Wang,&nbsp;Tianyi Wang,&nbsp;Hongge Qian,&nbsp;Xinghua Wei,&nbsp;Yaolong Yang,&nbsp;Lianguang Shang,&nbsp;Yue Feng","doi":"10.1111/jipb.13726","DOIUrl":null,"url":null,"abstract":"<p>Tiller angle is a key agricultural trait that establishes plant architecture, which in turn strongly affects grain yield by influencing planting density in rice. The shoot gravity response plays a crucial role in the regulation of tiller angle in rice, but the underlying molecular mechanism is largely unknown. Here, we report the identification of the <i>BIG TILLER ANGLE2</i> (<i>BTA2</i>), which regulates tiller angle by controlling the shoot gravity response in rice. Loss-of-function mutation of <i>BTA2</i> dramatically reduced auxin content and affected auxin distribution in rice shoot base, leading to impaired gravitropism and therefore a big tiller angle. BTA2 interacted with AUXIN RESPONSE FACTOR7 (ARF7) to modulate rice tiller angle through the gravity signaling pathway. The BTA2 protein was highly conserved during evolution. Sequence variation in the <i>BTA2</i> promoter of <i>indica</i> cultivars harboring a less expressed <i>BTA2</i> allele caused lower <i>BTA2</i> expression in shoot base and thus wide tiller angle during rice domestication. Overexpression of <i>BTA2</i> significantly increased grain yield in the elite rice cultivar Huanghuazhan under appropriate dense planting conditions. Our findings thus uncovered the BTA2-ARF7 module that regulates tiller angle by mediating the shoot gravity response. Our work offers a target for genetic manipulation of plant architecture and valuable information for crop improvement by producing the ideal plant type.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3000,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13726","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13726","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Tiller angle is a key agricultural trait that establishes plant architecture, which in turn strongly affects grain yield by influencing planting density in rice. The shoot gravity response plays a crucial role in the regulation of tiller angle in rice, but the underlying molecular mechanism is largely unknown. Here, we report the identification of the BIG TILLER ANGLE2 (BTA2), which regulates tiller angle by controlling the shoot gravity response in rice. Loss-of-function mutation of BTA2 dramatically reduced auxin content and affected auxin distribution in rice shoot base, leading to impaired gravitropism and therefore a big tiller angle. BTA2 interacted with AUXIN RESPONSE FACTOR7 (ARF7) to modulate rice tiller angle through the gravity signaling pathway. The BTA2 protein was highly conserved during evolution. Sequence variation in the BTA2 promoter of indica cultivars harboring a less expressed BTA2 allele caused lower BTA2 expression in shoot base and thus wide tiller angle during rice domestication. Overexpression of BTA2 significantly increased grain yield in the elite rice cultivar Huanghuazhan under appropriate dense planting conditions. Our findings thus uncovered the BTA2-ARF7 module that regulates tiller angle by mediating the shoot gravity response. Our work offers a target for genetic manipulation of plant architecture and valuable information for crop improvement by producing the ideal plant type.

Abstract Image

BTA2 通过控制辅助素的含量和分布来调节水稻的分蘖角度和芽的重力反应。
分蘖角度是建立植株结构的关键农业性状,而植株结构又通过影响水稻的种植密度对谷物产量产生强烈影响。芽的重力反应在水稻分蘖角度的调控中起着至关重要的作用,但其潜在的分子机制却大多不为人知。在此,我们报告了通过控制水稻的芽重力反应来调控分蘖角度的大分蘖角2(BTA2)的鉴定结果。BTA2 的功能缺失突变显著降低了水稻嫩枝基部的辅素含量并影响了辅素的分布,导致重力作用受损,从而导致大分蘖角。BTA2与AUXIN RESPONSE FACTOR7(ARF7)相互作用,通过重力信号通路调节水稻分蘖角度。BTA2 蛋白在进化过程中高度保守。在水稻驯化过程中,BTA2等位基因表达较低的籼型栽培品种的BTA2启动子序列变异导致BTA2在芽基中表达较低,从而导致分蘖角度变宽。在适当的密植条件下,BTA2 的过表达能显著提高水稻优良品种黄花占的谷粒产量。因此,我们的研究结果揭示了 BTA2-ARF7 模块通过介导芽重力反应来调节分蘖角度。我们的研究为植物结构的遗传操作提供了一个目标,并通过产生理想的植物类型为作物改良提供了有价值的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信