Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jiwei Mao , Hongyu Zhang , Yu Chen , Liang Wei , Jun Liu , Jens Nielsen , Yun Chen , Ning Xu
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引用次数: 0

Abstract

Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic changes has become an increasingly attractive approach for constructing robust microbial cell factories. In this review, we provide a brief overview of metabolic burden engineering, focusing specifically on recent developments and strategies for diminishing the burden while improving robustness and yield. A variety of examples are presented to showcase the promise of metabolic burden engineering in facilitating the design and construction of robust microbial cell factories. Finally, challenges and limitations encountered in metabolic burden engineering are discussed.

减轻代谢负担,提高工业微生物的稳健性和生物产量
代谢负担是指基因操作和环境扰动对细胞资源分配的影响。为生产生物基化学品而重新安排微生物新陈代谢往往会导致新陈代谢负担,随之而来的是不利的生理影响,如细胞生长受阻和产品产量低。减轻不良代谢变化带来的负担已成为构建稳健的微生物细胞工厂的一种越来越有吸引力的方法。在本综述中,我们将简要概述代谢负担工程,特别关注在提高稳健性和产量的同时减轻代谢负担的最新进展和策略。我们还列举了各种实例,以展示代谢负担工程在促进设计和构建稳健的微生物细胞工厂方面的前景。最后,还讨论了代谢负担工程中遇到的挑战和限制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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