[Effect of TLK2 Expression Regulated by MiR-21 on Proliferation and Apoptosis of Acute Myeloid Leukemia Cells].

Q4 Medicine

中国实验血液学杂志 Pub Date : 2024-06-01 DOI:10.19746/j.cnki.issn.1009-2137.2024.03.002

Bo Liang, Jun-Jie Yin, Sheng-Nan Zhang, Chao Zhang, Zi-Long Hu, Yi Wang

{"title":"[Effect of TLK2 Expression Regulated by MiR-21 on Proliferation and Apoptosis of Acute Myeloid Leukemia Cells].","authors":"Bo Liang, Jun-Jie Yin, Sheng-Nan Zhang, Chao Zhang, Zi-Long Hu, Yi Wang","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.002","DOIUrl":null,"url":null,"abstract":"Objective: To investigate the effect of TLK2 expression regulated by miR-21 on proliferation and apoptosis of acute myeloid leukemia cells.Methods: Seventy patients with AML admitted to our hospital from January 2019 to July 2022 were selected, while 30 patients with iron deficiency anemia were selected as the control group. Bone marrow mononuclear cells (BMMNCs) of the patients were obtained using Ficoll density gradient centrifugation. RT-qPCR was used to determine the expression levels of miR-21 and TLK2 mRNA in BMMNCs. Mimics-miR-21, mimics-NC, inhibitor-miR-21, inhibitor-NC and NC were transfected into HL-60 cells using liposome-mediated transfection technology. CCK-8 method was used to determine the activity of transfected HL-60 cells after treatment with cytarabine. The apoptosis rate of HL-60 transfected cells was determined by TUNEL method. The expression of TLK2 mRNA in HL-60 cells transfected with inhibitor-miR-21 was determined by RT-qPCR.Results: The relative expression levels of miR-21 and TLK2 mRNA in BMMNCs of AML patients were significantly higher than those of controls (both P < 0.05). After HL-60 cells were treated with cytarabine, both the cell activity of inhibitor-miR-21 group and mimics-miR-21 group decreased significantly with the increase of cytarabine concentration (both P < 0.05). However, at each concentration point of cytarabine, the cell activity of inhibitor-miR-21 group was lower than that of control group (P < 0.05), while mimics-miR-21 group was higher than control group (P < 0.05). After HL-60 cells were treated with cytarabine, the apoptosis rate of inhibitor-miR-21 group was significantly increased (P < 0.05), while that of mimics-miR-21 group was significantly decreased (P < 0.05). After HL-60 cells were treated with inhibitor-miR-21, the relative expression of TLK2 mRNA decreased significantly (P < 0.05).Conclusion: miR-21 is highly expressed in AML patients, which may promote the apoptosis of AML cells by inhibiting the expression of TLK2.","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实验血液学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To investigate the effect of TLK2 expression regulated by miR-21 on proliferation and apoptosis of acute myeloid leukemia cells.

Methods: Seventy patients with AML admitted to our hospital from January 2019 to July 2022 were selected, while 30 patients with iron deficiency anemia were selected as the control group. Bone marrow mononuclear cells (BMMNCs) of the patients were obtained using Ficoll density gradient centrifugation. RT-qPCR was used to determine the expression levels of miR-21 and TLK2 mRNA in BMMNCs. Mimics-miR-21, mimics-NC, inhibitor-miR-21, inhibitor-NC and NC were transfected into HL-60 cells using liposome-mediated transfection technology. CCK-8 method was used to determine the activity of transfected HL-60 cells after treatment with cytarabine. The apoptosis rate of HL-60 transfected cells was determined by TUNEL method. The expression of TLK2 mRNA in HL-60 cells transfected with inhibitor-miR-21 was determined by RT-qPCR.

Results: The relative expression levels of miR-21 and TLK2 mRNA in BMMNCs of AML patients were significantly higher than those of controls (both P < 0.05). After HL-60 cells were treated with cytarabine, both the cell activity of inhibitor-miR-21 group and mimics-miR-21 group decreased significantly with the increase of cytarabine concentration (both P < 0.05). However, at each concentration point of cytarabine, the cell activity of inhibitor-miR-21 group was lower than that of control group (P < 0.05), while mimics-miR-21 group was higher than control group (P < 0.05). After HL-60 cells were treated with cytarabine, the apoptosis rate of inhibitor-miR-21 group was significantly increased (P < 0.05), while that of mimics-miR-21 group was significantly decreased (P < 0.05). After HL-60 cells were treated with inhibitor-miR-21, the relative expression of TLK2 mRNA decreased significantly (P < 0.05).

Conclusion: miR-21 is highly expressed in AML patients, which may promote the apoptosis of AML cells by inhibiting the expression of TLK2.

查看原文本刊更多论文

[受 MiR-21 调控的 TLK2 表达对急性髓性白血病细胞增殖和凋亡的影响】。］

目的研究miR-21调控的TLK2表达对急性髓性白血病细胞增殖和凋亡的影响：选取我院2019年1月至2022年7月收治的70例急性髓系白血病患者作为研究对象，同时选取30例缺铁性贫血患者作为对照组。采用 Ficoll 密度梯度离心法获得患者的骨髓单核细胞（BMMNCs）。采用 RT-qPCR 方法测定 BMMNCs 中 miR-21 和 TLK2 mRNA 的表达水平。利用脂质体介导的转染技术将模拟物-miR-21、模拟物-NC、抑制物-miR-21、抑制物-NC 和 NC 转染到 HL-60 细胞中。用 CCK-8 法测定转染的 HL-60 细胞在接受阿糖胞苷处理后的活性。用 TUNEL 法测定转染 HL-60 细胞的凋亡率。用 RT-qPCR 法测定转染了抑制剂-miR-21 的 HL-60 细胞中 TLK2 mRNA 的表达：结果：AML患者BMMNCs中miR-21和TLK2 mRNA的相对表达水平明显高于对照组（P均<0.05）。HL-60细胞经阿糖胞苷处理后，随着阿糖胞苷浓度的增加，抑制剂-miR-21组和模拟物-miR-21组的细胞活性均明显下降（均P＜0.05）。然而，在阿糖胞苷的每个浓度点，抑制剂-miR-21 组的细胞活性均低于对照组（P＜0.05），而模拟物-miR-21 组则高于对照组（P＜0.05）。HL-60 细胞经阿糖胞苷处理后，抑制剂-miR-21 组的细胞凋亡率显著增加（P < 0.05），而模拟物-miR-21 组的细胞凋亡率显著降低（P < 0.05）。结论：miR-21在AML患者中高表达，可通过抑制TLK2的表达促进AML细胞的凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊