Adding yeast extract to culture medium enhances replication of the avian coronavirus infectious bronchitis virus in chicken embryo kidney cells

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-06-23 DOI:10.1016/j.jviromet.2024.114989

José A. Quinteros , Glenn F. Browning , Karim Mardani , Amir H. Noormohammadi , Mark A. Stevenson , Mauricio J.C. Coppo , Carlos A. Loncoman , Nino Ficorilli , Andrés Diaz-Méndez

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引用次数: 0

Abstract

Infectious bronchitis virus (IBV), an avian coronavirus, can be isolated and cultured in tracheal organ cultures (TOCs), embryonated eggs and cell cultures, the first two of which are commonly used for viral isolation. Previous studies have suggested that foetal bovine serum (FBS) can inhibit coronavirus replication in cell cultures. In this study, the replication of IBV in chicken embryo kidney (CEK) cell cultures and the Leghorn hepatocellular carcinoma (LMH) cell line was assessed using two different cell culture media containing FBS or yeast extract (YE) and two different IBV strains. The highest concentrations of viral genomes were observed when the cell culture medium (CEK) contained YE. Similar results were observed in LMH cells. Examination of the infectivity by titration demonstrated that the cell lysate from CEK cell cultures in a medium including YE contained a higher median embryo infectious dose than that from CEK cell cultures in a medium containing FBS. These results indicate that improved replication of IBV in cell cultures can be achieved by replacing FBS with YE in the cell culture medium.

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在培养基中添加酵母提取物可增强禽冠状病毒传染性支气管炎病毒在鸡胚肾细胞中的复制。

传染性支气管炎病毒（IBV）是一种禽类冠状病毒，可在气管器官培养物（TOC）、胚胎蛋和细胞培养物中分离和培养，前两种培养物通常用于病毒分离。以前的研究表明，胎牛血清（FBS）可抑制冠状病毒在细胞培养物中的复制。本研究使用含有 FBS 或酵母提取物（YE）的两种不同细胞培养基和两种不同的 IBV 株系，评估了 IBV 在鸡胚肾（CEK）细胞培养物和莱格霍恩肝细胞癌（LMH）细胞系中的复制情况。当细胞培养基（CEK）含有酵母提取物时，病毒基因组的浓度最高。在 LMH 细胞中也观察到了类似的结果。通过滴定法检测感染性表明，在含有 YE 的培养基中培养出的 CEK 细胞裂解物比在含有 FBS 的培养基中培养出的 CEK 细胞裂解物含有更高的中位胚胎感染剂量。这些结果表明，在细胞培养基中用 YE 替代 FBS 可以改善 IBV 在细胞培养物中的复制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.