In vitro effect of TiF4/NaF solution on the development of radiation-induced dentin caries.

IF 2.2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Applied Oral Science Pub Date : 2024-06-24 eCollection Date: 2024-01-01 DOI:10.1590/1678-7757-2024-0024

Beatriz Martines de Souza, Eduardo Lista Francisco, Aline Silva Braga, Paulo Sergio da Silva Santos, Marilia Afonso Rabelo Buzalaf, Ana Carolina Magalhães

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引用次数: 0

Abstract

Objective: To evaluate the protective effect of an experimental solution containing TiF4/NaF on the development of radiation-induced dentin caries lesions.

Methodology: bovine root samples were irradiated (70Gy) and distributed as following (n=12/group): Commercial Saliva (BioXtra), NaF (500 ppm F-), TiF4 (500 ppm F), TiF4/NaF (TiF4: 300 ppm F-, NaF: 190 ppm F-), and Phosphate buffer solution (PBS, negative control). Biofilm was produced using biofilm from irradiated patients and McBain saliva (0.2% of sucrose, at 37oC and 5% CO2) for five days. The treatments were applied 1x/day. Colony-forming units (CFU) were counted and demineralization was quantified by transversal microradiography. The ANOVA/Tukey test was applied for all parameters.

Results: All treatments reduced CFU for total microorganisms. TiF4 reduced Lactobacillus sp. (7.04±0.26 log10 CFU/mL) and mutans streptococci (7.18±0.28) CFU the most, when compared to PBS (7.58±0.21 and 7.75±0.17) and followed by NaF (7.12±0.31 and 7.34±0.22) and TiF4/NaF (7.16±0.35 and 7.29± 0.29). TiF4 and Commercial saliva showed the lowest integrated mineral loss (ΔZ-vol%.mm) (1977±150 and 2062±243, respectively) when compared to PBS (4540±335), followed by NaF (2403±235) and TiF4/NaF (2340±200). Commercial saliva was the only to significantly reduce mineral loss (LD-µm) (111±25) compared to PBS (153±24).Mean mineral loss (R-vol%) decreased by 35.2% for TiF4 (18.2±3.3) when compared to PBS (28.1±2.9) Conclusion: TiF4/NaF has a comparable anti-cariogenic effect to TiF4 and Commercial saliva under the model in this study.

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TiF4/NaF 溶液对辐射诱发牙本质龋齿发展的体外效应。

目的评估含有 TiF4/NaF 的实验溶液对辐射诱导的牙本质龋损发展的保护作用。方法：牛根部样本经辐照（70Gy）后按以下方式分配（n=12/组）：商用唾液（BioXtra）、NaF（500 ppm F-）、TiF4（500 ppm F）、TiF4/NaF（TiF4：300 ppm F-，NaF：190 ppm F-）和磷酸盐缓冲溶液（PBS，阴性对照）。使用辐照患者的生物膜和麦克贝恩唾液（蔗糖含量为 0.2%，温度为 37 摄氏度，二氧化碳含量为 5%）制作生物膜，为期五天。每天处理 1 次。对菌落形成单位（CFU）进行计数，并通过横向微射线照相术对脱矿情况进行量化。所有参数均采用方差分析/Tukey 检验：结果：所有处理方法都降低了总微生物的 CFU。与 PBS（7.58±0.21 和 7.75±0.17）和 NaF（7.12±0.31 和 7.34±0.22）以及 TiF4/NaF（7.16±0.35 和 7.29±0.29）相比，TiF4 对乳酸杆菌（7.04±0.26 log10 CFU/mL）和变异链球菌（7.18±0.28）CFU 的减少最多。与 PBS（4540±335）相比，TiF4 和商业唾液的综合矿物质流失量（ΔZ-vol%.mm）最低（分别为 1977±150 和 2062±243），其次是 NaF（2403±235）和 TiF4/NaF（2340±200）。与 PBS（153±24）相比，商业唾液是唯一能明显减少矿物质流失（LD-µm）（111±25）的物质。与 PBS（28.1±2.9）相比，TiF4（18.2±3.3）的平均矿物质流失（R-vol%）减少了 35.2%：在本研究的模型中，TiF4/NaF 的抗龋齿效果与 TiF4 和商业唾液相当。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.