Ayami Ota, Takahiro Iguchi, Sachiko Nitta, Ryunosuke Muro, Nanami Mino, Masayuki Tsukasaki, Josef M Penninger, Takeshi Nitta, Hiroshi Takayanagi
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引用次数: 0
Abstract
The thymus is an organ required for T cell development and is also an eosinophil-rich organ; however, the nature and function of thymic eosinophils remain unclear. Here, we characterized the gene expression and differentiation mechanism of thymic eosinophils in mice. Thymic eosinophils showed a distinct gene expression profile compared with other organ-resident eosinophils. The number of thymic eosinophils was controlled by medullary thymic epithelial cells (mTECs). In Rag-deficient mice, the unique gene expression signature of thymic eosinophils was lost but restored by pre-T cell receptor signalling, which induces CD4+ CD8+ thymocyte differentiation, indicating that T cell differentiation beyond the CD4- CD8- stage is necessary and sufficient for the induction of thymic eosinophils. These results demonstrate that thymic eosinophils are quantitatively and qualitatively regulated by mTECs and developing thymocytes, respectively, suggesting that thymic eosinophils are a distinct, thymus-specific cell subset, induced by interactions with thymic cells.
胸腺是 T 细胞发育所需的器官,也是嗜酸性粒细胞丰富的器官;然而,胸腺嗜酸性粒细胞的性质和功能仍不清楚。在这里,我们研究了小鼠胸腺嗜酸性粒细胞的基因表达和分化机制。与其他器官驻留的嗜酸性粒细胞相比,胸腺嗜酸性粒细胞显示出独特的基因表达谱。胸腺嗜酸性粒细胞的数量由胸腺髓质上皮细胞控制。在 Rag 缺失的小鼠中,胸腺嗜酸性粒细胞的独特基因表达特征消失了,但通过诱导 CD4+ CD8+ 胸腺细胞分化的前 T 细胞受体信号恢复了这一特征。这些结果表明,胸腺嗜酸性粒细胞在数量和质量上分别受到髓质胸腺上皮细胞和发育中胸腺细胞的调控,这表明胸腺嗜酸性粒细胞是一种独特的胸腺特异性细胞亚群,是通过与胸腺细胞相互作用诱导的。
期刊介绍:
International Immunology is an online only (from Jan 2018) journal that publishes basic research and clinical studies from all areas of immunology and includes research conducted in laboratories throughout the world.