Differential gene expression and gut microbiota composition in low-altitude and high-altitude goats

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Zhenzhen Zhang , Xin Zhang , Tingting Zhang , Jingjing Li , Cuomu Renqing , Zhaxi Baijiu , Sangzhu Baima , Wangjie Zhaxi , Yuzhen Nima , Wangsheng Zhao , Tianzeng Song
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引用次数: 0

Abstract

Previous studies have presented evidence suggesting that altitude exerts detrimental effects on reproductive processes, yet the underlying mechanism remains elusive. Our study employed two distinct goat breeds inhabiting low and high altitudes, and conducted a comparative analysis of mRNA profiles in testis tissues and the composition of gut microbiota. The results revealed a reduced testis size in high-altitude goats. RNA-seq analysis identified the presence of 214 differentially expressed genes (DEGs) in the testis. These DEGs resulted in a weakened immunosuppressive effect, ultimately impairing spermatogenesis in high-altitude goats. Additionally, 16S rDNA amplicon sequencing recognized statistically significant variations in the abundance of the genera Treponema, unidentified_Oscillospiraceae, Desulfovibrio, Butyricicoccus, Dorea, Parabacteroides between the two groups. The collective evidence demonstrated the gut and testis played a synergistic role in causing decreased fertility at high altitudes. Our research provides a theoretical basis for future investigations into the reproductive fitness of male goats.

低海拔和高海拔山羊的基因表达和肠道微生物群组成差异。
以往的研究有证据表明,海拔高度会对生殖过程产生不利影响,但其潜在机制仍然难以捉摸。我们的研究采用了栖息在低海拔和高海拔地区的两个不同山羊品种,并对睾丸组织的 mRNA 图谱和肠道微生物群的组成进行了比较分析。结果显示,高海拔山羊的睾丸体积缩小。RNA-seq分析确定了睾丸中存在214个差异表达基因(DEGs)。这些差异表达基因导致免疫抑制作用减弱,最终损害了高海拔山羊的精子发生。此外,16S rDNA 扩增子测序发现,在两组山羊之间,特雷波纳菌属、不明弧菌属、去硫弧菌属、丁酸球菌属、多雷菌属、副乳菌属的丰度存在显著的统计学差异。综合证据表明,肠道和睾丸在导致高海拔地区生育能力下降方面起着协同作用。我们的研究为今后研究公山羊的生殖健康提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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