Biotechnological potential of psychrotolerant methylobacteria isolated from biotopes of Antarctic oases

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-06-22 DOI:10.1007/s00203-024-04056-7

Dmitry Yuryevich Sharavin, Polina Gennadievna Belyaeva

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Abstract

Ten strains of psychrotolerant methylotrophic bacteria were isolated from the samples collected in Larsemann and Bunger Hills (Antarctica). Most of the isolates are assigned to the genus Pseudomonas, representatives of the genera Janthinobacterium, Massilia, Methylotenera and Flavobacterium were also found. Majority of isolates were able to grow on a wide range of sugars, methylamines and other substrates. Optimal growth temperatures for the isolated strains varied from 6 °C to 28 °C. The optimal concentration of NaCl was 0.5–2.0%. The optimal pH values of the medium were 6–7. It was found that three strains synthesized indole-3-acetic acid on a medium with L-tryptophan reaching 11–12 μg/ml. The values of intracellular carbohydrates in several strains exceeded 50 μg/ml. Presence of calcium-dependent and lanthanum-dependent methanol dehydrogenase have been shown for some isolates. Strains xBan7, xBan20, xBan37, xBan49, xPrg27, xPrg48, xPrg51 showed the presence of free amino acids. Bioprospection of Earth cryosphere for such microorganisms has a potential in biotechnology.

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从南极绿洲生物群落中分离的精神耐受性甲基细菌的生物技术潜力。

从（南极洲）拉塞曼山和邦格山采集的样本中分离出了 10 株精神耐受性滋养甲基细菌。大部分分离物被归入假单胞菌属，也发现了 Janthinobacterium、Massilia、Methylotenera 和 Flavobacterium 属的代表。大多数分离菌株都能在多种糖类、甲基胺和其他底物上生长。分离菌株的最佳生长温度从 6 °C 到 28 °C 不等。氯化钠的最佳浓度为 0.5-2.0%。培养基的最佳 pH 值为 6-7。研究发现，三株菌株在含有 L-色氨酸的培养基上合成的吲哚-3-乙酸达到 11-12 μg/ml。一些菌株的细胞内碳水化合物含量超过了 50 μg/ml。一些分离菌株存在钙依赖型和镧依赖型甲醇脱氢酶。菌株 xBan7、xBan20、xBan37、xBan49、xPrg27、xPrg48、xPrg51 显示存在游离氨基酸。在地球低温层对这类微生物进行生物勘探具有生物技术方面的潜力。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.